| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,567 | 0 | T | G | 60.0% | 8.7 / 13.2 | 15 | G81G (GGT→GGG) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCC > NZ_CP009273/2089481‑2089653 | aCCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGgggggg > 2:360677/1‑90 (MQ=255) gCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGggggggggt > 1:635996/1‑87 (MQ=255) gCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAATTTCTGGAAg > 1:282360/1‑90 (MQ=255) aaaGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGGCTCTGGGAGATGGCGC‑GGAAAATc > 1:475574/1‑90 (MQ=255) gggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGTCTCTGGGAGATGGCGCGGAAAATTTTTTct > 1:185963/1‑89 (MQ=255) gggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGAAGCCCC‑GAAAAATTTTTTCt > 2:284641/1‑90 (MQ=255) tcGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGgcgc < 2:201074/90‑1 (MQ=255) tcGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGAAGGCGC‑GGAAAATTTTTTCTTTTgcgc > 1:290486/1‑90 (MQ=255) cgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTTGCGCGGa > 2:77692/1‑90 (MQ=255) ttCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACtctc > 2:137058/1‑90 (MQ=255) gTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTTCTTTTGCGCGGGTAAAATTTTCATCAACTCTCCTgc > 2:622762/1‑90 (MQ=255) gTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGAAGCCCC‑GAAAATTCTTTTCTTTTGCGCGGGTAAAAATTCCATCAACTCTCCTgc > 2:504093/1‑90 (MQ=255) tttGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTgcgc < 2:342204/90‑1 (MQ=255) ttGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGATGCCGC‑GAAAATTTTTTTCTTTGGGGCGGGTAAAAATTTCATCAACTCTCCTTCGCt > 1:84632/1‑90 (MQ=255) gtgGCGGGTGGGATTAAGTCTCTGGAAAATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCa < 1:710643/90‑1 (MQ=255) gggTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAccc < 2:380358/90‑1 (MQ=255) gggTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAccc < 2:417026/90‑1 (MQ=255) | ACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCC > NZ_CP009273/2089481‑2089653 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |