| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,141,214 | 0 | T | G | 62.5% | 4.5 / 13.5 | 16 | G20G (GGT→GGG) | yegD | molecular chaperone |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (10/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGC > NZ_CP009273/2141134‑2141302 | cACCGGCATGGGGATTATTTCGCGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCAt < 1:313766/90‑1 (MQ=255) gCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGGGGCGGGAAAACGCCtttttt > 2:346094/1‑87 (MQ=255) ggggATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGGCATGCGTGGCGGGGAAACCCACTTGCTtaaa > 2:438310/1‑90 (MQ=255) ttattTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGGCGGGAAAACCCATTTTTTAAAAAAGGa > 2:399601/1‑90 (MQ=255) attTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGaaa < 1:49586/90‑1 (MQ=255) cAGCAAACTGTTCAGTGGCGGTCATGCGTGGCGGGAAAACGCATTTTCTAAAAAAGGAAAAAGACAGCACGCTGCTGCCTTCAATGCttt > 2:219585/1‑90 (MQ=255) cAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGGAAACCGCATTTGCTAAAAAAGGAAAAAGACAGCACGCTGCTGCCTTCAATGCttt > 2:228261/1‑90 (MQ=255) gCAAACTGTTCAGTGGCGGTCATGCGGGGCGGGAAACCGCCTTTTTTAAAAAAGGAAAAAGAAAGCACGCCGCCTCCCTCAATGCttttc > 1:160300/1‑88 (MQ=255) aaaCTGTTCAGTGGCGGTCATGCGTGGCGGGAAACCCCATTTGCTAAAAAAGGAAAACGACAGCACGCCGCCGGCCTCAAAGCtttttgc > 1:173692/1‑86 (MQ=255) aaCTGTTCAGTGGCGGTCATGCGTGACGGGAAACCGCATTTTCTAAAAAAGGAAAAAGACAGCACCCTGCCGCCCTCAATGCTTTtcgcg > 1:248264/1‑90 (MQ=255) aaCTGTTCAGTGGCGGTCATGCGTGACGGGAAAACCCATTTTCTAAAAAAGGAAAAAGACAGCACGCTGCTGCCTTCCATTCTTTtcgcg > 2:712944/1‑90 (MQ=255) tCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAAcg < 1:455980/90‑1 (MQ=255) tCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAAcg < 1:470367/90‑1 (MQ=255) gCGGTCATGCGTGACGGTAAACCGCATTTTCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGaa > 2:183305/1‑90 (MQ=255) ggTCATGCGGGACGGGAAAACGCATTTGCTAAAAATGGAAAAAGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAAAGCGGGAAGc > 1:273283/1‑90 (MQ=255) gTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGc < 2:239232/90‑1 (MQ=255) | CACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGC > NZ_CP009273/2141134‑2141302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |