| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,678,659 | 0 | A | C | 58.3% | 6.2 / 10.3 | 12 | Y70D (TAT→GAT) | glyA | serine hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (0/7); total (4/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGC > NZ_CP009273/2678575‑2678748 | cGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGc > 1:525915/1‑90 (MQ=255) gcgcCGAACAGTTCTTTCGCACGATCGATCGGCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATaa > 2:575547/1‑90 (MQ=255) gcgcCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATaa > 1:217070/1‑90 (MQ=255) aaaCATTTTTTTCCCCCGATCGATCCCCATTTTTTCAACGATATCAACATACCCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTc < 2:147499/89‑1 (MQ=255) ttcttcCGCCCGATCGTCCGCCATTTTTCAAACAATATCAACATCCCCCCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCata < 1:144429/90‑1 (MQ=255) ttCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATttg > 2:363618/1‑90 (MQ=255) cgatcgagCGCCATTTTTTAAACTATACCAACATCCCCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 2:389861/90‑1 (MQ=255) cgatcgacCCCCAGTTGTCCAACGATATCAACATCCCCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 2:21236/90‑1 (MQ=255) cgCCATTTTTCCAAAAATATAAACATCCCCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTgaga < 2:577257/90‑1 (MQ=255) ttttttCAACAATACCAACATCCCCACACCCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCt < 1:681230/86‑1 (MQ=255) ttCAACGATATCAACATCCTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTgcgc < 2:68740/90‑1 (MQ=255) aCATCCCCGCACCCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTAcgcgc < 2:611739/90‑1 (MQ=255) aCTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGc < 2:217070/90‑1 (MQ=255) | CGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGC > NZ_CP009273/2678575‑2678748 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |