| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,852,477 | 0 | G | A | 54.5% | 1.8 / 19.6 | 22 | E676K (GAA→AAA) | mutS | DNA mismatch repair protein MutS |
| Reads supporting (aligned to +/- strand): ref base G (6/4); new base A (0/12); total (6/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGAT > NZ_CP009273/2852393‑2852560 | ggACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAAcc > 2:590114/1‑90 (MQ=255) tttCCCCGCGTGGGCGCGGAAGATGACCTGGCGCCCGGGCCCTCACCCTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAAc < 2:555903/90‑1 (MQ=255) tccccGGGTGGGGGCGGAAGATGCCCGGGCGCCCGGGCCCCCACCTTTTAGGGGGGAGATGAATAAAACCGCCAATATTTTACATAACGc < 1:313642/88‑1 (MQ=255) tccccGCGTGGGCGCGGAAGATGCCCGGGCGTCGGGCCCCCCACCTTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGc < 1:162858/88‑1 (MQ=255) tccccGCGAGGGCGGGGCAGTCCCCCGGGCGCCGGGGCCCCAAACTTTTCTGGGGGATAGGACTGAACCCGCCAATATTTTACATAACGc < 2:635015/88‑1 (MQ=255) cccGCGTGGGGGCGGCAGATGCCCGGGCGTCGGGGCGCTCACCTTTTAGGGGGGAGATGACAAAAACCGCCAATATTTTACATAACGCCa < 2:438662/90‑1 (MQ=255) ccGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCACCCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCAc < 2:135390/90‑1 (MQ=255) cgGCAGATGACCTGGCGTCGGGGCCCTCACCCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTc < 2:97278/90‑1 (MQ=255) cgGCAAATGCCCGGGCGCCGGGGCCCTCACCTTTTTGGGGGGAGATGATTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTc < 1:150118/90‑1 (MQ=255) ggCAGATGCCCGGGCGCCCGGGCCCTCACCTTTTATGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCt < 1:144417/90‑1 (MQ=255) ggCAGATGACCTGGCGTCCGGGCCCACACCTTTTATGGGGGAGATGCCGAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCt < 2:173327/90‑1 (MQ=255) gCAGATGCCCTGGCGCCCGGGCCCTCACCTTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTg < 2:566416/90‑1 (MQ=255) cAGATGCCCGGGCGTCGGGGCGCCCAACCTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTgg < 2:308474/90‑1 (MQ=255) gATGCCCGGGCGCCGGGGCGCTCAACCTTTAGGGGGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGgtg < 1:286686/90‑1 (MQ=255) tGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATgg > 1:291044/1‑90 (MQ=255) ccTTTAGGGGGGAGATGACGAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGaa < 1:128166/90‑1 (MQ=255) tggAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc > 1:676504/1‑71 (MQ=255) tggAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCt > 1:699364/1‑90 (MQ=255) gggAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc < 2:676504/70‑1 (MQ=255) ggAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTa > 1:723279/1‑90 (MQ=255) ggAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTa > 1:55308/1‑90 (MQ=255) aTGACTAAACCCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGAt < 2:465595/90‑1 (MQ=255) | GGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGAT > NZ_CP009273/2852393‑2852560 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |