| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 294,671 | 0 | A | C | 60.0% | 3.9 / 15.3 | 15 | Y659D (TAT→GAT) | paoC | aldehyde oxidoreductase molybdenum‑binding subunit PaoC |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/9); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCG > NZ_CP009273/294590‑294739 | tCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCa > 1:275657/1‑90 (MQ=255) ggggAGGATGGGGGGTCGTTATCACCCAGAAAAACCCCCTCTTTTTTTGGGATATCGGGATGACCCGCCCCCTCATCCCCCGCCATATCg < 1:76736/90‑1 (MQ=255) gaTATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTa > 1:160430/1‑90 (MQ=255) gaTATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTa > 1:498522/1‑90 (MQ=255) tggggggTGGGTATCACCAAGAAAAACCCCCTCCTTTTTTGGGATATCCGCATGACCCGCCCCCTCATCCCCCGCCATATCGTGATTAAc < 1:107984/87‑1 (MQ=255) gggTCGGTACCCCCAAGAAAAACCCCCCCTTTTTTTGGGATATCCGCATGACCCGCCCCCTCATCCCCCGCCATATCGTGATTAACGAAg < 1:100665/90‑1 (MQ=255) ggTGGGTACCACCCAGAAAAACCCCCCCCTGTTTTGGGATACCCGCATGACCCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGt < 2:82320/90‑1 (MQ=255) cGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGc > 1:324723/1‑90 (MQ=255) atcaccCAGAAAAACCCCCCCCTTTTTGGGGATACCCGAATGACCCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCaa < 2:501121/90‑1 (MQ=255) tcCAGAAAAACCCCCTCCTTTTTTGGGATACCCGCATGACCCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACgg < 2:403167/90‑1 (MQ=255) ccccctTTTTGGGGATACCCGAATGACCCGCCCCCCCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCa < 2:512485/87‑1 (MQ=255) tcctGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGc > 1:373820/1‑90 (MQ=255) gggATACCCGCATGACCCGGCCCCCCACCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATc < 1:103007/90‑1 (MQ=255) tatCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTg > 2:527662/1‑90 (MQ=255) ccacATGACCCGCCCCCCCACCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCcg < 1:16512/87‑1 (MQ=255) | TCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCG > NZ_CP009273/294590‑294739 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |