| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 371,857 | 0 | T | G | 53.8% | 5.3 / 11.9 | 13 | L315V (TTG→GTG) | mhpT | 3‑(3‑hydroxy‑phenyl)propionate transporter |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (7/0); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCGGCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGTGTCGTCATTTAACGGTATGTTGCTGGCGGGATTTGTCGCGGGGTTGTTTGCGACAGGTGGGCAAAGCGTTTTGTATGCCCTGGCACCGTTGTTTTACAGTTCGCAGATCCGCGCAACAG > NZ_CP009273/371776‑371932 | aGCGGCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGTGTCGTCATTTAACGGTATGTTGCTGGCGGGGTTTGTTGCGGGGGTGTTTGCg > 1:536687/1‑90 (MQ=255)aGCGGCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGTGTCGTCATTTAACGGTATGTTGCTGGCGGGGGTTTTTGCGGGGGTGGTTGCg > 1:539907/1‑90 (MQ=255) ggCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGTGTCGTCATTTAACGGTATGTTGCTGGCGGGATTTGTCGCGGGgttgtt < 1:152425/83‑1 (MQ=255) ggCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGTGTCGTCATTTAACGGTATGTTGCTGGCGGGATTTGTCGCGGGgttgtt > 2:152425/1‑83 (MQ=255) ggCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGTGTCGTCATTTAACGGTATGTTGCTGGCGGGATTTGTCGCGGGGGTGTTTGGGACa > 2:275836/1‑90 (MQ=255) ggCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGGGTCGTCATTTAACGGTATGTTGCTGGCGGGATTTGTGGCGGGGGTGTTTTGGACa > 1:678364/1‑90 (MQ=255) gCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGTGTCGTCATTTAACGGTATGTTGCTGGCGGGATTTGTCGCGGGGGTGTTTGCGACAg > 2:74055/1‑90 (MQ=255) ttCGCTGCTGGCGCTTGGAACGGTGTCGTCATTTAACGGTATGTTGCTGGCGGGATTTGTCGCGGGGTTGTTTGCGACACGTGGGCAAAg < 1:601885/90‑1 (MQ=255) aaCGGTGTCGTCATTTAACGGTATGTTGCTGGCGGGATTTGTCGCGGGGTTGTTTGCGACAGGTGGGCAAAGCGTTTTGTATGCCCTGGc < 1:607849/90‑1 (MQ=255) tgtCGTCATTTAACGGTATGTTGCTGGCGGGATTTGTCGCGGGGTTGTTTGCGACAGGTGGGCAAAGCGTTTTGTATGCCCTGGCACCgt < 2:275246/90‑1 (MQ=255) ggTATGTTGCTGGCGGGATTTTTCGCGGGGGTGTTTTCGACAGGTGGGCAAAACGTTTTGTTTGCCCTGGCCCCGGTGTTTTTAAGtttg > 1:580051/1‑88 (MQ=255) gTATGTTGCTGGCGGGATTTGTCGCGGGGTTGTTTGCGACAGGTGGGCAAAGCGTTTTGTATGCCCTGGCACCGTTGTTTTACAGTTCGc > 2:198289/1‑90 (MQ=255) gTATGTTGCTGGCGGGATTTGTCGCGGGGGTGTTTGCGACAGGTGGGCAAAGCGTTTTGTATGCCCTGGCACCGTTGTTTTACAGTTCGc > 2:121298/1‑90 (MQ=255) ttGCTGGCGGGATTTGTCGCGGGGTTGTTTGCGACAGGTGGGCAAAGCGTTTTGTATGCCCTGGCACCGTTGTTTTACAGTTCGCAGATc > 1:213673/1‑90 (MQ=255) gATTTGTCGCGGGGTTGTTTGCGACAGGTGGGCAAAGCGTTTTGTATGCCCTGGCACCGTTGTTTTACAGTTCGCAGATCCGCGCAACAg < 1:561874/90‑1 (MQ=255) | AGCGGCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGTGTCGTCATTTAACGGTATGTTGCTGGCGGGATTTGTCGCGGGGTTGTTTGCGACAGGTGGGCAAAGCGTTTTGTATGCCCTGGCACCGTTGTTTTACAGTTCGCAGATCCGCGCAACAG > NZ_CP009273/371776‑371932 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |