Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,455,3940CT54.5% 1.3 / 11.4 11S82F (TCC→TTC) paaJbifunctional 3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base T (6/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGGCTGTGCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCTGCCGCAGAGTGTTTCCGGCACAACCATT‑AACCGCTTGTGTGGTT‑CCGGGCTGGACGCACTGGGGTTT  >  NZ_CP009273/1455311‑1455446
                                                                                   |                                                      
cGGCTGTGCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGGTGCCGGGAAGGGTTTTCGGcac                                                  >  2:550396/1‑90 (MQ=255)
cGGCTGTGCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCTGCCGCAGAGTGTTTCCGGcac                                                  <  2:169609/90‑1 (MQ=255)
cGGCTGTGCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCTGCCGCAGAGTGTTTCCGGcac                                                  <  2:674942/90‑1 (MQ=255)
     gtgCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGGGGGCGCCGAGGGGTTTCCGCACAAccc                                             >  1:571701/1‑89 (MQ=255)
     gtgCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCGGCCCCGGGGGGTTTTCCGCACAAccc                                             >  2:586624/1‑89 (MQ=255)
     gtgCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCCGCCGCAGAGGGTTTTCGGCCCAACCa                                             >  2:21927/1‑90 (MQ=255)
              atgCGGGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCTGCCGCAGAGTGTTTCCGGCACAACCATT‑AACCGCt                                   <  2:268154/88‑1 (MQ=255)
                             aCCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCTGCCGCAGGATGGTTTCGGCGCCAACATTAAACCGC‑TGTGTTGTTGCCggg                    >  2:298278/1‑90 (MQ=255)
                                 tAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCTGCCGCAGAGTGTTTCCGGCACAACCATT‑AACCGCTTGTGTGGTT‑CCGGGCTGGa               <  2:571701/90‑1 (MQ=255)
                                   aCGTAGCCCGGATGGCGACTTTACTGGCGGGGGTGCCGCCGAGGGGTTTCCGCCCAACCACT‑AACCCCTTGTGTGGGT‑TCGGGGTGGGCg             >  1:281583/1‑90 (MQ=255)
                                              aTGGCGACTTTACTGGCGGGGCTGCCGCAGAGTGTTTCCGGCACAACCATT‑AACCGCTTGTGTGGTT‑CCGGGCTGGACGCACTGGGGttt  <  1:100301/90‑1 (MQ=255)
                                                                                   |                                                      
CGGCTGTGCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCTGCCGCAGAGTGTTTCCGGCACAACCATT‑AACCGCTTGTGTGGTT‑CCGGGCTGGACGCACTGGGGTTT  >  NZ_CP009273/1455311‑1455446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑