| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,561,995 | 0 | A | T | 58.3% | 2.2 / 10.9 | 12 | V362V (GTT→GTA) | digH | glycoside hydrolase family 10 protein |
| Reads supporting (aligned to +/- strand): ref base A (1/4); new base T (7/0); total (8/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCT‑GGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCACTCCGTGAGAACGGCCAGTAAATTTGGGGAGC > NZ_CP009273/1561919‑1562072 | aGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCT‑GGTCGGTTTAACGACATCCGccc < 1:72471/90‑1 (MQ=255) gTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGGTGGGTTTAA‑GACATCCGcccc > 2:217140/1‑89 (MQ=255) aTCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCT‑GGGCGGTTTTACCACATCCCCCCCCCCttttttc > 2:252964/1‑87 (MQ=255) tttATAGAAGGCGATACCGATATACAGGCGGGTCCT‑GGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGc < 1:169246/90‑1 (MQ=255) tataGAAGGCGATACCGATATACAGGCGGGTCCT‑GGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCAc < 2:379566/90‑1 (MQ=255) aaGGCGATACCGATATACAGGCGGGGCCT‑GGTCGGGTTTAAGACAACCGCCCCCCCCTTTTTCCACACCCCATAAACCCCGGCAcccccc > 2:561833/1‑88 (MQ=255) aGGCGATACCGATATACAGGCGGGGCCT‑GGTCGGGGTTAAGACAACCCCCCCCCACTTTTCCCACACGTCCTAAACCGCGGCACTccccg > 2:406592/1‑87 (MQ=255) ggCGATACCGATATACAGGCGGGGCCC‑GGGCGGGTTTAAAAAAACCCCCCCCCCTTTTTTCAACACCCCATAAAGCGCGGCACCCCGTga > 2:652306/1‑90 (MQ=255) gATATACAGGCGGGGGCT‑GGGGGGGTTTACGACAACCCCCCCCCCCTTTTCCCACACCACATAAAGCGCGGCCCCCCCCGGGAACGGCCa > 1:188381/1‑90 (MQ=255) atatACAGGCGGGGCCT‑GGGCGGGTTTAAGACAACCCCCCCCCCTTTTTCCCACACGTCATAACCCGCGGGACCCCCCGAGAAAGGcccg > 2:8938/1‑88 (MQ=255) ggggCCT‑GGGCGGGTTTAAGACATCCCCCCCCCCTTTTTCCAACACGTCATAAAGCGCGGGCCCCCGCGGGAAAGGCCAGGAAAATTtgg > 1:209787/1‑90 (MQ=255) ccT‑GGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCACTCCGTGAGAACGGCCAGTAAATTTGGGGAGc < 1:43064/90‑1 (MQ=255) | AGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCT‑GGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCACTCCGTGAGAACGGCCAGTAAATTTGGGGAGC > NZ_CP009273/1561919‑1562072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |