Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,370,6300AC54.5% 6.0 / 13.2 11Y148D (TAT→GAT) menH2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base C (0/6);  total (3/8)
Fisher's exact test for biased strand distribution p-value = 6.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CAAGCGTTGCGCCATTATTGTTGCTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGT  >  NZ_CP009273/2370543‑2370712
                                                                                       |                                                                                  
cAAGCGTTGCGCCATTATTGTTGCTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATAcc                                                                                  <  1:623433/90‑1 (MQ=255)
              ttattGTTGCTGCGCAGCGCCCCCACCCCCCGGCGTTGATCGTCATTGAGGGAGGAAAAAACAGGCTGTTGATCCCAGTCGGAAAATAcc                                                                    <  1:59993/90‑1 (MQ=255)
                     tgctgcGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTa                                                             >  2:633187/1‑90 (MQ=255)
                                           ccGGCTTTGTTCGTCATGGGGGGAGAAAAACCCGGGCTGTTGATCCCCGCCGGAAAACCCCGCCGTTAACGGTTCTGTGGGAAAACGCTg                                       <  1:424560/90‑1 (MQ=255)
                                             ggCGTTGATCGTCATTGGGTGAGGAAAAAACAGGCTGTTGATCCCAGTCGGAAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCa                                     <  1:580116/90‑1 (MQ=255)
                                                       gTCATTGAGTGAGGAAAAAACGGGCTGTTGATCCCAGCCGGAAAATCCCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCg                           <  1:361999/90‑1 (MQ=255)
                                                        tCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGa                          >  1:225189/1‑90 (MQ=255)
                                                                                tGTTGATCCCAGTCGGAAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACgt  <  2:62430/90‑1 (MQ=255)
                                                                                tGTTGACCCCAGCCGACAAATCCCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACgt  <  1:633187/90‑1 (MQ=255)
                                                                                 gTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACgt  >  1:94191/1‑89 (MQ=255)
                                                                                 gTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACgt  <  2:94191/89‑1 (MQ=255)
                                                                                       |                                                                                  
CAAGCGTTGCGCCATTATTGTTGCTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGT  >  NZ_CP009273/2370543‑2370712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑