| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,636,048 | 0 | T | G | 54.5% | 5.6 / 10.6 | 11 | A52A (GCA→GCC) | rodZ | cytoskeleton protein RodZ |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (6/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGGCAGCAGTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGTGCCTTATCTTCTTCAATGTCGCGT‑ACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCG > NZ_CP009273/2635960‑2636137 | cTGGCAGCAGTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGTg < 2:222791/90‑1 (MQ=255)cTGGCAGCAGTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGTg < 2:398330/90‑1 (MQ=255) agTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGTGCCTTAtct < 1:134810/90‑1 (MQ=255) agTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGTGCCTTAtct < 2:265884/90‑1 (MQ=255) tGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGGGGCCTATTTTTTTCTAtctc > 2:275787/1‑90 (MQ=255) cAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGGGGCCTTTTTTTTTTAATTTCGCGG‑ACCggggg > 2:156969/1‑89 (MQ=255) cgcgCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCCTTTTTTTTTTC‑ATTTTGCGT‑ACCCTGGgaaac > 1:651904/1‑88 (MQ=255) cGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGTGCCTTATTTTCTTCCATGTTGCGG‑ACCGGGGGAACCTTCAGGCaaaat > 2:528615/1‑88 (MQ=255) atCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGGGGCTTTTTTTTTTTTATTTTGCGTGACCGGGGAAACC‑TCCGGCAAAAACGCCCgg > 1:151848/1‑90 (MQ=255) cgcAGGAATGTTGAAGCAAGATCGGC‑GGGGGGCTTTTTTTTTTTCATTTCGCGG‑ACCGGGGGAAACTTCAGGCAAAAAGGCTCGGCCAcc > 2:433875/1‑89 (MQ=255) cgcAGGAATGTTGAAGCAAGATCGGC‑GGGGGCCTTTTCTTTTTCAATGTCGCGT‑ACCGTGGGAACCCTTCGGCAAAGACGCTCGGccaag > 2:78214/1‑86 (MQ=255) tGCCTTATCTTCTTCAATGTCGCGT‑ACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTcgcg < 1:427764/90‑1 (MQ=255) tGCCTTATCTTCTTCAATGTCGCGT‑ACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTcgcg < 2:65733/90‑1 (MQ=255) | CTGGCAGCAGTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGTGCCTTATCTTCTTCAATGTCGCGT‑ACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCG > NZ_CP009273/2635960‑2636137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |