| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,994,529 | 0 | A | C | 53.8% | 7.1 / 12.9 | 13 | T289P (ACC→CCC) | xdhA | xanthine dehydrogenase molybdenum‑binding subunit XdhA |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAA‑GTTT‑CCC‑TTAGCCGTGAA‑GAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTG > NZ_CP009273/2994440‑2994609 | agCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAA‑GTTT‑CCC‑TTAGCCGTGAA‑GAGTGTTTCCTCGCAACCCGTa > 1:187340/1‑90 (MQ=255)agCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAA‑GTTT‑CCC‑TTAGCCGTGAA‑GAGTGTTTCCTCGCAACCCGTa > 1:467402/1‑90 (MQ=255) gCAAGCTTGGCGGCATTCCGGTGAAA‑GTTT‑CCC‑TTAGCCGTGAA‑GAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACg > 2:84070/1‑90 (MQ=255) gCAAGCGGGGCGGC‑TTCCGGGAAAATCTTT‑CCC‑TTGGCGGTGAA‑GTGTTTTCCCTCGCACCCCGTCCCCGCCACGCTTTTACCATTGACg < 1:38486/90‑1 (MQ=255) gggcggc‑TTCCGGGTAAA‑TTTTCCCC‑TTAGCGGTGAA‑GGTTTTTCCCCCGCACCCCGTCCCCGCCACGCTTTTATCATTGACGGGCAAAt < 2:336284/89‑1 (MQ=255) aattt‑CCC‑TTGGCCCGGAAGGTGTTTTCCCCCCCACCCCGCCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACg < 1:485547/88‑1 (MQ=255) tt‑CCCTTTAGCGGTGAG‑GTGTTTTCCCCCGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGaa < 1:158893/90‑1 (MQ=255) tAGCCGTGAA‑GAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGaa > 1:659722/1‑90 (MQ=255) aGCGGTGAA‑GAGTTTTCCCCCCCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGaaa < 2:114003/90‑1 (MQ=255) aGCGGTGAA‑GAGTTTTCCCCCCCACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGaaa < 2:170916/90‑1 (MQ=255) aGCGGTAAA‑GGGTTTTCCCCCCCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGaaa < 1:246542/90‑1 (MQ=255) gtgtTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCt > 2:209186/1‑90 (MQ=255) tttCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGa > 2:381236/1‑90 (MQ=255) ccGCACCCCCTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTc < 1:60153/89‑1 (MQ=255) gCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTg > 2:102422/1‑90 (MQ=255) | AGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAA‑GTTT‑CCC‑TTAGCCGTGAA‑GAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTG > NZ_CP009273/2994440‑2994609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |