| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,168,596 | 0 | G | T | 61.1% | 5.6 / 18.5 | 18 | R54S (CGC→AGC) | parE | DNA topoisomerase IV subunit B |
| Reads supporting (aligned to +/- strand): ref base G (5/2); new base T (0/11); total (5/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGTATAC > NZ_CP009273/3168528‑3168681 | cACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGc > 2:119435/1‑90 (MQ=255) cGGCACCCCGCCCCCATCGAAAATACCTTCAAACGACTGGTCAGAATAAAAAAAACCGCCCCCGCTTTTTGCGTGACCCGCCAGTGCTTc < 1:305284/90‑1 (MQ=255) tCCCCCCCCCATCGTAAATATCTTTTACCGACGGGTCACCATGAAAAATAACGCCCACGGTTTTTGCGTTCCCCGCCAGTGCTTCATCca < 1:173477/90‑1 (MQ=255) cccGCCCCCATCGTCAATACCTTCAAACGACTGGTCAGCATAAAAAATAACGCCCACGCTTTTTGCGTGACCCGCCAGTGCTTCATCcac < 1:19247/90‑1 (MQ=255) cgccCCCATCGTAAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCCCGCTTTTTGCGTGACCCGCCAGTGCTTCATCCacac < 2:479031/90‑1 (MQ=255) cccccccATCGTCAATACCTCCTAACGACTGGTCAGAATAAAAAATACCGTCCCCGCTTTTTGGGTGCCCCGCCAGTGCTTCATCCacac < 1:386711/86‑1 (MQ=255) gcgcCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGGGCTTCATCCACACt > 1:406749/1‑90 (MQ=255) gcccccATGGTCAATAATTTCAAACGACTGTTCAGCATGAAAAAAAACGCCCACGCTTTTGGCGTGACCCGCCAGTGCTTCATCCACACt < 1:275458/87‑1 (MQ=255) aaaTACCTTTTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGa < 2:30022/89‑1 (MQ=255) tCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGc > 1:665500/1‑90 (MQ=255) tCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGc > 2:632988/1‑90 (MQ=255) cgtcTGCTCAGCATGAAAAATAACGTCCCCGGTTTTTGCGTCACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCGCaa < 1:11898/87‑1 (MQ=255) cGACTGGTCAGCATGAAAAAAACCGCCCCCGCTTTTTGCGTGCCCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCaa < 2:510077/90‑1 (MQ=255) gACTGGTCAGCATGTAAAATAACGTCCCCGCTTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCaaa < 2:133662/90‑1 (MQ=255) ggTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGt > 1:363550/1‑90 (MQ=255) tgaaaaaTACCGCCCCCGGTTTTTGCGTGCCCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGa < 1:464667/87‑1 (MQ=255) accGTCCACGCTTTTTGCGTGCCCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATc < 2:591553/88‑1 (MQ=255) ccgctTTTTGCGTGCCCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGTATAc < 2:642035/89‑1 (MQ=255) aCGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGTATAc < 2:56091/90‑1 (MQ=255) | CACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGTATAC > NZ_CP009273/3168528‑3168681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |