| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,781,385 | 0 | T | G | 57.1% | 8.1 / 21.2 | 21 | G396G (GGT→GGG) | envC | murein hydrolase activator EnvC |
| Reads supporting (aligned to +/- strand): ref base T (5/4); new base G (12/0); total (17/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAGTTTTGTTTCCATT > NZ_CP009273/3781298‑3781471 | cTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCg > 2:258037/1‑90 (MQ=255) taATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGcc < 2:323742/90‑1 (MQ=255) gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCCGGGGCGGccctccct > 1:491103/1‑90 (MQ=255) gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCAcc > 2:385299/1‑89 (MQ=255) aCTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTAttt < 2:488318/90‑1 (MQ=255) gTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTTGaa > 2:446754/1‑90 (MQ=255) gCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTTGAAATTc > 2:352632/1‑90 (MQ=255) ttGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCCCTCTATTTCGAAATTcgcc > 2:399323/1‑90 (MQ=255) ggctCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCCTCACTCTATTTTGAAATTcgccgc > 1:435537/4‑90 (MQ=255) ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCCTCCCTCCATTTCGAAATTcgcccc > 1:407093/1‑88 (MQ=255) tCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGGCAggg > 2:194131/1‑89 (MQ=255) tCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCAGGGGCGGCCTTTACTCGCTTTTGGAATTTGCCGCCAGGGGCAggg > 1:132049/1‑89 (MQ=255) cagggCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc < 2:519521/88‑1 (MQ=255) ttGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGGCAGGCGGTCAATCCACAGCCGt > 2:201566/1‑90 (MQ=255) agTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGGTGGGAAGATAAGtt > 2:309378/1‑90 (MQ=255) agTGGCGGTCAGGGGCGGCCTTCCCTCTATTTTGAAATTCGCCGCCAGGGGCAGGCGGGCAATCCACAGCCGGGGTTGGGGAGATAAGtt > 2:478113/1‑90 (MQ=255) agTGGCGGTCAGGGGCGGCCTTCCCTCTATTTCGAAATTTGCCGCCCGGGGCAGGGGGGCAATCCCCCGCCGTGGGTGGGGAGATAAggt > 1:591164/1‑88 (MQ=255) ggCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAGTTTTg > 2:53128/1‑90 (MQ=255) gggTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAGTTTTGTTTCCAtt > 2:151481/1‑90 (MQ=255) gTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc > 1:529247/1‑47 (MQ=255) gTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc < 2:529247/47‑1 (MQ=255) | CTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAGTTTTGTTTCCATT > NZ_CP009273/3781298‑3781471 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |