| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 240,196 | 0 | A | C | 53.8% | 2.4 / 12.0 | 13 | H56P (CAT→CCT) | lpcA | D‑sedoheptulose 7‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base C (7/0); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CACGCCATTCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGAGTTGACCGGTCGCTACCGTGAAAACCGTCCGGGCTACCCGGCGATTGCT > NZ_CP009273/240111‑240272 | cACGCCATTCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGGTCCCCTTGc > 2:23756/1‑90 (MQ=255) cGCCATTCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGa < 1:121789/90‑1 (MQ=255) ttCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGGTTCCCTTTCGgcgcca > 1:60730/1‑88 (MQ=255) cgcgGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACtt < 1:666281/90‑1 (MQ=255) aGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGGTCCCCTTTCGGCGCCATGCACTTTTCCCGAGAGATGACCgg > 1:100078/1‑90 (MQ=255) aGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCCTTGCGACGCTATGCACTTTGCCGAAGAGGTGACCCGgcgc > 2:607837/1‑90 (MQ=255) aGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGAGGTGACCGGTCGc > 2:168036/1‑90 (MQ=255) cGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCCTTTCGGCGCTATGCACTTTTCCGAAGGGTTGACCGGGCGCTACCGGGaaaa > 2:34149/1‑90 (MQ=255) cGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCCTTTCGGCGCCATGCACTTTTCCCAAGAGGTGACCGGGCGCTACCCTGaaaa > 1:172294/1‑90 (MQ=255) cGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACCCTATGCACTTTTCCCAAAAGGTTACCCGTCCCCACCCGGaaaa > 1:321732/1‑90 (MQ=255) gTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGAGTTGACCGGTCGCTACCGTGAAAACCGTCCGGGc < 1:545825/90‑1 (MQ=255) gCGGCAACGGCGGGTCCCCTTGCGACGCTATGCACCTTTTCGCAGAGTTGACCGGGCGCCCCCCTGAAAAACCGCCGGGCCTCCCcgcgg > 1:516894/1‑89 (MQ=255) aaCGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGAGTTGACCGGTCGCTACCGTGAAAACCGTCCGGGCTACCCGGCGATTGCt < 1:122460/90‑1 (MQ=255) | CACGCCATTCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGAGTTGACCGGTCGCTACCGTGAAAACCGTCCGGGCTACCCGGCGATTGCT > NZ_CP009273/240111‑240272 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |