Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 49,905 T→G L28R (CTC→CGC)  folA → type 3 dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927349,9050TG97.6% 131.8 / NA 41L28R (CTC→CGC) folAtype 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (19/21);  minor base C (1/0);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49820‑49991
                                                                                     |                                                                                      
tcAATGATCAGTCTGATTGCGGCGTTGGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGcc                                                                                    <  1:200534/90‑1 (MQ=255)
tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGcc                                                                                    <  1:276391/90‑1 (MQ=255)
tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGcc                                                                                    <  1:637258/90‑1 (MQ=255)
 cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCt                                                                                   <  1:272995/90‑1 (MQ=255)
        cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaa                                                                            >  2:260190/1‑90 (MQ=255)
         aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                                                                           >  2:394755/1‑90 (MQ=255)
         aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGGTTaaa                                                                           >  2:595199/1‑90 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  2:91157/90‑1 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  1:245288/90‑1 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  1:49354/90‑1 (MQ=255)
                   cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCt                                                                 <  2:525539/90‑1 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGGACCCGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                                                >  1:12208/1‑90 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                                                >  2:76798/1‑90 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGGTTAAACGCAACACCtt                                                                >  1:426703/1‑90 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGAACCGGCCTGGTTTAAAAGCAACACCtt                                                                >  1:628338/1‑90 (MQ=255)
                         tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTtaaata                                                           <  1:148385/90‑1 (MQ=255)
                               tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg                                                     >  2:497716/1‑90 (MQ=255)
                                aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                                                    <  2:110362/90‑1 (MQ=255)
                                 gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTg                                                   >  2:639129/1‑90 (MQ=255)
                                   tCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGAt                                                 >  1:273135/1‑90 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                               >  2:196434/1‑90 (MQ=255)
                                     gcgTTATCGGCATGGAAAACCCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                               >  2:334468/1‑90 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                            >  1:502291/1‑90 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                            >  1:40326/1‑90 (MQ=255)
                                           tCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      >  1:547567/1‑61 (MQ=255)
                                           tCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  2:547567/61‑1 (MQ=255)
                                           ccgtcATGGAAAACGCCATGCCGGGAAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgcc                                         <  1:530595/86‑1 (MQ=255)
                                             ggCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                       <  1:260190/90‑1 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  1:568602/56‑1 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      >  2:568602/1‑56 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                    >  1:218732/1‑90 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                    <  2:135657/90‑1 (MQ=255)
                                                aTGAAAAACGCCAGGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                    <  1:537785/90‑1 (MQ=255)
                                                                   ggACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc                 <  1:413757/90‑1 (MQ=255)
                                                                   ggAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc                 <  2:224375/90‑1 (MQ=255)
                                                                   ggAACCGGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc                 <  2:242393/90‑1 (MQ=255)
                                                                       cctgcctgCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc             >  1:500136/1‑90 (MQ=255)
                                                                        ctgcctgcCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg            >  1:470083/1‑90 (MQ=255)
                                                                         tgcctgccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt           >  1:410282/1‑90 (MQ=255)
                                                                                  aTCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc  <  2:515294/90‑1 (MQ=255)
                                                                                  aTCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc  <  2:653999/90‑1 (MQ=255)
                                                                                     |                                                                                      
TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49820‑49991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: