| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 700,041 | 0 | T | G | 58.8% | 10.3 / 14.2 | 17 | G214G (GGT→GGG) | nagE | PTS N‑acetyl glucosamine transporter subunit IIABC |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base G (10/0); total (14/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTG > NZ_CP009273/699958‑700129 | aCCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTTCACGGGGACAtt > 2:34224/1‑90 (MQ=39) cTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTCCACGGGGACATTAAcccc > 2:286746/1‑88 (MQ=39) aGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACCGGGACATTAACCGCTTCTAtt > 1:523169/1‑89 (MQ=255) cTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACCGGTTTCCCCCGTGGCCTTTACCGCCTCTCTGCCggg > 1:580108/1‑89 (MQ=255) ccATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGTTTTCCCCGGGGACATTAACCGCTTCTATGCCGGGggcggcg > 1:268867/1‑89 (MQ=255) ccATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTCCCCGGGGGCATTAACCCCCTCTATCCCCGCggcggct > 1:239805/1‑89 (MQ=255) cGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACcgc < 1:590819/90‑1 (MQ=255) gCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGTTTTCCACGGGGACATTAACCGCTTCTATGCCGGTGACGGCACcgcg > 2:480042/1‑90 (MQ=255) gTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTTCCCGGGGACATTAACCGCTTCTTTTCCGGGGACGGGCCCGCgggggg > 1:602615/1‑88 (MQ=255) gATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCAt < 1:91942/90‑1 (MQ=255) tGGTGAATTCACCAACGCGGCGGGGACGGGTTTCCACGGTGACATTAACCGCTTCTATGCCGGGGACGGCACCGCGGGGGTGTTCATGTc > 1:212463/1‑90 (MQ=255) ggTGAATTCACCAACGCGGCGGGTACGGTTTTTCACGGGGACATTAACCGCTTCTATGCCGGGGGCGGCCCCGCGGGGGTGTTCATGTcc > 1:32463/1‑90 (MQ=255) ggTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGGGGCATTAAACGCTTCTATGCCGGGGGCGGCACCGCGGGGGTGTTCATTTcc > 1:117665/1‑90 (MQ=255) aTTCACCAACGCGGCGGGTACGGGTTTCCACGGGGACATTAACCGCTTCTATGCCGGGGACGGCACCGCGGGGGTGTTCATGTCCGGctt > 2:179876/1‑90 (MQ=255) gcggGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCatg < 2:32463/90‑1 (MQ=255) gTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTg > 2:626277/1‑90 (MQ=255) gTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTg > 2:640379/1‑90 (MQ=255) | ACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTG > NZ_CP009273/699958‑700129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |