| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,963 | 0 | T | G | 64.7% | 2.1 / 14.8 | 17 | D492A (GAC→GCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (11/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.08e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT > NZ_CP009273/822877‑823052 | ccATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCaa < 2:105970/90‑1 (MQ=255) cATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAc < 1:84120/90‑1 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGTGGGGGGCAACCCCgcgaa > 1:18720/1‑86 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGCACGCCGGaga > 1:539357/1‑88 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGCGAGGGGGGGAAAGCCGGGAg > 1:599387/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 1:61243/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCCACGCCGGAAGGCGGGTCGTCGAAAAAACGAAAGGCGGGTTCCTGc > 1:459486/1‑90 (MQ=255) cTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCGACCCCGGGAGACGGGTCGGCGGGAAAAAAAAAGTCCGGGTCATGCATc > 1:77844/1‑90 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg < 2:258000/90‑1 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAGTCGGGTTGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAg > 2:61243/1‑90 (MQ=255) tGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAGGGGGTTCGTCGGGAAAAAGAAAGTCCGGTTCATGCATCcgcg > 1:526547/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTCGTCGAGAAACAGAAAGTCCGGGTCATGCATCAGCGAACAgg > 1:600926/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGAACAgg > 2:180728/1‑90 (MQ=255) aGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTCGGCGAGAAAAAGAAAGTCCGGGTCATGCAACAGCGGACAGgcc > 1:463342/1‑90 (MQ=255) aGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGTTCATCGAGAAACAGAATGTCCGGGTCATGCATCAGCGGACAgggc > 2:251224/1‑88 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:575672/90‑1 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGCCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:568137/90‑1 (MQ=255) tCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAt > 2:183369/1‑90 (MQ=255) | CCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT > NZ_CP009273/822877‑823052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |