| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,567 | 0 | T | G | 59.1% | 11.0 / 19.0 | 22 | G81G (GGT→GGG) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base T (4/5); new base G (13/0); total (17/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAAC > NZ_CP009273/2089478‑2089656 | atCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGt < 2:102046/90‑1 (MQ=255) cTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTa < 1:380001/90‑1 (MQ=255) ttCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGttta > 1:96676/1‑88 (MQ=255) ttCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTaa > 2:169105/1‑90 (MQ=255) tGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGTTTTTGGa > 1:508649/1‑90 (MQ=255) tGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTctcttgg > 2:608656/1‑87 (MQ=255) gtgtGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGTTCTGGGAgggg > 1:593808/1‑87 (MQ=255) aGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑Gaa < 1:340194/90‑1 (MQ=255) aGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAAGCTCTGGGAGGTGGcgc‑gga > 1:408043/1‑88 (MQ=255) aGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCtt < 2:426862/90‑1 (MQ=255) tGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTcc > 2:237401/1‑90 (MQ=255) gggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGCGGAAAATTTTTTct > 1:80333/1‑89 (MQ=255) tctcGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGC‑GAAAATTTTTTTCttttgg > 2:102763/1‑87 (MQ=255) ggAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAATCTCCGGGAGATGCCCC‑GGAAATTCTTTCCCTTTGCGCGGAGaaaaa > 2:291862/1‑89 (MQ=255) gATCGACATTCCGTTTTGGGTGGCGGGTGGGGGTGAGGGCCTGGAAGAAGTCCC‑GCAAATTTTTTTCTTTTGCGCGGGGAAAAATTcctt > 1:573350/1‑88 (MQ=255) tCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAAGCCTTGGTGGATGGCCC‑GTAAATTTTTTTCTTTTGCGGGGATAAAATTTTCCTCa > 2:490887/1‑90 (MQ=255) gACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAAc < 2:295444/90‑1 (MQ=255) ttCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCttt < 2:542324/57‑1 (MQ=255) ttCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGC‑GAAAAATTTTTTCTTTTGCGCGGATAAAAATTTCATCAAAtctc > 2:582979/1‑90 (MQ=255) ttCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCttt > 1:542324/1‑57 (MQ=255) ttGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTTGCGCGGGTAAAATTTCCATCAACTCTCCTGCGCt > 2:529835/1‑90 (MQ=255) ttGTGTAGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCt > 2:418576/1‑90 (MQ=255) gCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTTCATCAACTCTCCTGCGCTGGCAGAc > 2:568596/1‑90 (MQ=255) tGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAAc < 2:577584/90‑1 (MQ=255) | ATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAAC > NZ_CP009273/2089478‑2089656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |