| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,738,448 | 0 | A | C | 57.9% | 4.6 / 16.7 | 19 | V84G (GTG→GGG) | trmD | tRNA (guanosine(37)‑N1)‑methyltransferase TrmD |
| Reads supporting (aligned to +/- strand): ref base A (4/4); new base C (0/11); total (4/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCC > NZ_CP009273/2738359‑2738525 | gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa > 1:238094/1‑90 (MQ=255) acacCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTg < 2:584450/90‑1 (MQ=255) aaTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAACCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCAccc > 1:176544/1‑90 (MQ=255) tGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCcgcg < 1:185100/90‑1 (MQ=255) gTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCtttt > 2:332124/1‑90 (MQ=255) gCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTtgct < 2:306425/90‑1 (MQ=255) gCTGCCCCCCGCTTGACCAAGCTTGCCCCCTGGTGGTGAAAAAAAAACCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAAt < 1:321517/90‑1 (MQ=255) aCGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCg > 1:226019/1‑90 (MQ=255) cGCCCGTTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCTTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGt < 2:566413/90‑1 (MQ=255) cGCCCGCTTGATCAAGCTTGCGTCCTTGGGGGGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGt < 1:314093/90‑1 (MQ=255) gCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTc > 2:201314/1‑90 (MQ=255) cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa < 2:64869/90‑1 (MQ=255) cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa < 2:302720/90‑1 (MQ=255) ttGATCAACCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCaa < 2:176544/90‑1 (MQ=255) ctcgCGTCCCTGTGGTGACAGATAAATCCCCTTTGCCCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc < 1:40146/87‑1 (MQ=255) cTTGCGTCCCTGTTGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc < 2:462330/90‑1 (MQ=255) cTTGCGTCCCTGTGGTGACAAATAAATCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc < 1:332124/90‑1 (MQ=255) tgtgGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAAc < 1:44818/90‑1 (MQ=255) tgtgGTGACAGAAAAATCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAAc < 1:587337/90‑1 (MQ=255) gACAAATAAACCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc < 2:477649/90‑1 (MQ=255) | GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCC > NZ_CP009273/2738359‑2738525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |