| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,978,077 | 0 | G | C | 60.0% | 2.0 / 10.3 | 10 | P292R (CCG→CGG) | yqeF | acetyl‑CoA C‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/3); new base C (6/0); total (7/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCG‑CAATTCCCATCAATGCCGGATCTACA‑CCAACGCTGGCAAATGCGCGAATGCGGGCCAGCA‑CGGGTAAATTCAACGCTCGT > NZ_CP009273/2978009‑2978160 | ccTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCG‑CAATTCCCATCAATGCCg < 1:100574/90‑1 (MQ=255) gACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCCGCGCCAATCCCCCCCAAACCCGG‑TCTACA‑CCCAc > 1:627427/1‑90 (MQ=255) aCTGCCAGCCTACACGCTCCTGGCAACGGCGGGGCGCATACACCCGCG‑CAATTCCCCTCAAAGCCGGGTCTACA‑CCAACGCTGGCAAATg > 2:29394/1‑90 (MQ=255) cGCTCCAGGCTACGGCGGGTCGCATACACCGGCG‑CAATTCCCATCAATGCCGGATCTACA‑CCAACGCTGGCAAATGCGCGAATGCGGGcc < 2:541520/90‑1 (MQ=255) ccAGGCAACGGCGGGGCGCATACACCCGCG‑CAATTCCCCTCCATGCCCGATCTACACCCACCCCCGGCAAA‑GCGCGAAAGCGGGGCAGcc > 1:541520/1‑89 (MQ=255) cggcggGTCGCATACACCCGCG‑CAAATCCCCTCAAAGCCGGGTCTACA‑CCCACCCTGGCAAAAGCGCGAAAGCGGGGCCGCA‑CGGGGaaa > 1:134634/1‑90 (MQ=255) gggTCGCATACACCCGCG‑CAATTCCCCTCAATGCCGGATCTACA‑CCAACGCTGGCAAAAGCGCGGAAGCGGGCCCGCACCGGGAAAAtta > 1:155347/1‑89 (MQ=255) ggTCGCATACACCCGCG‑CAATTCCCATCAATGCCGGGTCTACA‑CCAACCCTGGCAAAAGCGCGAAAGCGGGCCCGCA‑CGGGGAAATTTaa > 2:264756/1‑90 (MQ=255) ccGGCG‑CAATTCCCATCAATGCCGGATCTACA‑CCAACGCTGGCAAATGCGCGAATGCGGGCCAGCA‑CGGGTAAATTCAACGCTCGt < 1:141578/86‑1 (MQ=255) ccGGCG‑CAATTCCCATCAATGCCGGATCTACA‑CCAACGCTGGCAAATGCGCGAATGCGGGCCAGCA‑CGGGTAAATTCAACGCTCGt > 2:141578/1‑86 (MQ=255) | CCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCG‑CAATTCCCATCAATGCCGGATCTACA‑CCAACGCTGGCAAATGCGCGAATGCGGGCCAGCA‑CGGGTAAATTCAACGCTCGT > NZ_CP009273/2978009‑2978160 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |