| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 108,789 | 0 | T | C | 59.1% | 8.2 / 19.1 | 22 | S100G (AGC→GGC) | zapD | cell division protein ZapD |
| Reads supporting (aligned to +/- strand): ref base T (6/3); new base C (0/13); total (6/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.38e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGAGTTT > NZ_CP009273/108706‑108870 | gATGCTCAGTCGCTGACGCCCCAAAGAAATCAAACGATTTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgc < 2:426831/90‑1 (MQ=255)gATGCTCAGTCGCTGACGCACCAGAGAAATCAAACGATCTCCACGCAAAAATTGCCCGATACGCGGCGCGAAAATTAATACGCCCCccgc < 1:257403/90‑1 (MQ=255) cACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAAt > 2:252422/1‑90 (MQ=255) ggagCAACAAAACAATCTCCCCGCAAAATTTCCCCGATACGGGGCGGGAAAATTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAAt < 1:230699/89‑1 (MQ=255) agagCAATCAAACGATCTCCCCCCAAAAATTGCCCAATCCGCGGCGCGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAAt < 1:484247/90‑1 (MQ=255) agagCAATAAAACGATCTTCACGCAAAAATTCCCCGATCCGCGGCGGGAAAATTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAAt < 1:210008/90‑1 (MQ=255) aaacAAACGATCTCCACGCAAAATTTCCCCGATCCGGGGCGCGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTc < 1:423451/87‑1 (MQ=255) aaTCAAATGCTCTTCCCGCAAAAATTGCCCAATACGCGGCGCGCAAATTAATCCCCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTc < 2:344448/90‑1 (MQ=255) aTCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCa > 2:572950/1‑90 (MQ=255) aaCGATCTTCCCGCAGAAATTGCCCGATCCGCGGCGGGGAAATTAATACGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc < 2:163296/90‑1 (MQ=255) aaCGATCTCCCCGCAAAAATTGCCCGATACGCGGCGCGAAAATTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc < 1:252422/90‑1 (MQ=255) aaCGATCTCCCCGAAAAAATTCCCCGATACGCGGCGCGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc < 2:414570/90‑1 (MQ=255) aCGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACg > 2:393474/1‑90 (MQ=255) tctcCACGAAAAAATTCCCCGATCCGCGGCGCGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGctc < 1:156681/90‑1 (MQ=255) tCTTCACGCAAAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGctc < 2:37506/90‑1 (MQ=255) cTTCACGCAAAAATTCCCCGATCCGCGGCGCGAAAATTAATACGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGctct < 2:1588/90‑1 (MQ=255) aCGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTc > 1:186688/1‑90 (MQ=255) tccccGATGCGCGGCGCGGAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGc < 2:585547/88‑1 (MQ=255) gCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCa > 1:167633/1‑90 (MQ=255) ccGATACGCGGCGGGAAAATAAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACg < 1:45939/90‑1 (MQ=255) aaataaatCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGa < 1:90676/90‑1 (MQ=255) tAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGACTGGAGttt > 2:288719/1‑90 (MQ=255) | GATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGAGTTT > NZ_CP009273/108706‑108870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |