Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,283,874	Δ1 bp	intergenic (‑350/‑105)	BW25113_RS11435 ← / → narP	IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,283,871	0	T	.	100.0%	23.7 / NA	7	intergenic (‑347/‑108)	BW25113_RS11435/narP	IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (2/5);  total (2/5)

TTTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGC  >  NZ_CP009273/2283783‑2283913                                                                                         |                                           ttttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGtt                                           <  1:393993/90‑1 (MQ=255)        gttgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAG‑TTTCACTAc                                   <  2:25066/90‑1 (MQ=255)          tgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAG‑TTTCACTACAt                                 <  1:561417/90‑1 (MQ=255)           gttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGtttc                                         >  1:575354/1‑81 (MQ=255)           gttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGtttc                                         <  2:575354/82‑2 (MQ=255)               ttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAG‑TTTCACTACATGTCca                            <  2:21013/90‑1 (MQ=255)                              tGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAG‑TTTCACTACATGTCCATACATAAAATGGGGt             <  2:542568/90‑1 (MQ=255)                                      aaTTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAG‑TTTCACTACATGTCCATACATAAAATGGGGTAACATTCa     >  2:341094/1‑90 (MQ=255)                                      aaTTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAG‑TTTCACTACATGTCCATACATAAAATGGGGTAACATTCa     >  2:344194/1‑90 (MQ=255)                                         tCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAG‑TTTCACTACATGTCCATACATAAAATGGGGTAACATTCAcgc  <  1:151149/90‑1 (MQ=255)                                                                                         |                                           TTTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGC  >  NZ_CP009273/2283783‑2283913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑