Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,634,151	Δ1 bp	coding (164/534 nt)	rrrQ ←	lysozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,634,150	0	G	.	100.0%	88.8 / NA	22	coding (165/534 nt)	rrrQ	lysozyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (11/11);  total (11/11)

TTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAA  >  NZ_CP009273/1634064‑1634235                                                                                       |                                                                                      ttATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACtgtgcac                                                                                    >  1:581845/1‑87 (MQ=255)      aCGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCGTATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGa                                                                              >  2:457167/1‑90 (MQ=255)        gCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACa                                                                            >  1:109261/1‑90 (MQ=255)        gCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACa                                                                            >  1:456557/1‑90 (MQ=255)                  gTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCat                                                                  <  2:520331/90‑1 (MQ=255)                  gTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCat                                                                  >  2:490863/1‑90 (MQ=255)                  gTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCat                                                                  <  1:495980/90‑1 (MQ=255)                   ttGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCata                                                                 <  2:489730/90‑1 (MQ=255)                     gACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACtgtgca                                                                                     <  1:292217/69‑3 (MQ=255)                     gACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACtgtgca                                                                                     >  2:292217/1‑67 (MQ=255)                     gACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCATATg                                                               >  2:46187/1‑90 (MQ=255)                          ggTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCATATGCCAGa                                                          >  1:370114/1‑90 (MQ=255)                          ggTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCATATGCCAGa                                                          >  1:104202/1‑90 (MQ=255)                          ggTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCATATGCCAGa                                                          >  2:401855/1‑90 (MQ=255)                                  ttttCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCCCCCCGACAGATGGTCCATATGCCAGAACCATcgc                                                  <  1:358747/90‑1 (MQ=255)                                      ccTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTa                                              <  2:109261/90‑1 (MQ=255)                                       cTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTAt                                             <  2:52106/90‑1 (MQ=255)                                               gTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATtgt                                     >  1:404406/1‑90 (MQ=255)                                                  tCATATTGGGAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATtgtgtg                                  <  2:245669/90‑1 (MQ=255)                                                         gggAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACct                           >  2:371098/1‑90 (MQ=255)                                                         gggAAAAACGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACct                           >  1:385700/1‑90 (MQ=255)                                                                 cGGTTTTTCCATCCACCACTGT‑GCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTc                   <  1:46187/90‑1 (MQ=255)                                                                 cGGTTTTTCCATCCACCACTGT‑CCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTc                   <  1:464643/90‑1 (MQ=255)                                                                         ccatccaCCACTGT‑GCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGaa           <  2:370114/90‑1 (MQ=255)                                                                                  acTGT‑GCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCaa  <  2:509684/90‑1 (MQ=255)                                                                                       |                                                                                      TTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAA  >  NZ_CP009273/1634064‑1634235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑