| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,934,995 | +C | intergenic (‑308/‑173) | yieP ← / → BW25113_RS19520 | FadR/GntR family transcriptional regulator/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,934,991 | 1 | . | C | 96.0% | 70.4 / ‑2.6 | 25 | intergenic (‑304/‑177) | yieP/BW25113_RS19520 | FadR/GntR family transcriptional regulator/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (13/11); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01 | |||||||||||
GAACTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACA‑CCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGAT > NZ_CP009273/3934928‑3935075 | gaACTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAaaagcaaa > 2:346432/1‑90 (MQ=255) cTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTg > 1:414728/1‑75 (MQ=255) cTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTg < 2:414728/75‑1 (MQ=255) cTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCg > 1:94805/1‑90 (MQ=255) gCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACt < 1:320609/90‑1 (MQ=255) gCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACt < 2:483625/90‑1 (MQ=255) gaAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGctct > 2:144907/1‑90 (MQ=255) gaAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGctcc > 2:448197/1‑89 (MQ=255) aaaaTAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACaa < 1:138462/90‑1 (MQ=255) cTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGAc < 1:360763/90‑1 (MQ=255) cTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGAc > 2:586400/1‑90 (MQ=255) cTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGAc < 2:273256/90‑1 (MQ=255) ctGTAGCGGGAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCtgt > 2:257777/1‑90 (MQ=255) gggAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCa > 1:218857/1‑90 (MQ=255) gggAAAGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCa > 1:62799/1‑90 (MQ=255) aaaGCGTATTATGCACACCCCCGCGCCGCTGAGAAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACt > 2:408282/1‑90 (MQ=11) aaaGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTc < 2:122600/90‑1 (MQ=255) aaaGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTc < 2:109574/90‑1 (MQ=255) aGCGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGa > 2:110256/1‑90 (MQ=255) aGCGTATTATGCACA‑CCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGaa > 2:74553/1‑90 (MQ=35) cGTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGCCACTCGAAg < 1:44809/90‑1 (MQ=11) gTATTATGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGa > 2:248856/1‑90 (MQ=255) tattatGCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGAt < 2:430597/90‑1 (MQ=255) atGCACACCCCCGCGCCGCTGAGAAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACg < 1:408282/90‑1 (MQ=11) gCACACCCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGAt > 2:452106/1‑90 (MQ=255) | GAACTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACA‑CCCCGCGCCGCTGAG‑AAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGAT > NZ_CP009273/3934928‑3935075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |