| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,622,316 | 0 | T | G | 64.3% | 4.8 / 11.8 | 14 | G181G (GGT→GGG) | ydfG | bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (9/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGA‑GTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAG > NZ_CP009273/1622237‑1622401 | gTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGA‑Gt > 2:218185/1‑90 (MQ=255) gCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGA‑GTTTTCCAATg < 1:198582/90‑1 (MQ=255) gCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGGGCGCGTCACCGACAACGAACCGGGGCTGGGGGGGGGGACCGAGGTTTTCCatg > 1:378636/1‑88 (MQ=255) gCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGA‑GTTTTCCAATGTCCGCTTTaa < 2:273269/90‑1 (MQ=255) aCGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGG‑GGTTTCCCATGTCCGCTTTTAAggg > 1:338349/1‑89 (MQ=255) aTCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGA‑GTTTTCCAATGTCCGCTTTAAAAGCGATg < 1:472950/90‑1 (MQ=255) gCATGGTACGGCGGTGCGCGTCACCCACATCGAAACGGGGCTGGGGGGGGGGACCGG‑GGTTTTCAATGTCCGCTTTTAAGGCGGTGGCgg > 1:353817/1‑90 (MQ=255) gTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGTGGGGGGGACCGA‑GGTTTCCAATGTCCGCTTTAAAGGCGATGACGGGAAAg > 1:518353/1‑90 (MQ=255) gTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGA‑GGTTTTCAATGTCCCCTTTTAAGGGGGTGGCGGTAAAg > 1:113487/1‑90 (MQ=255) gcggTGCGCGTCACCGACATCGAACCGGGGCTGGTGGGGGGGACCGA‑GTTTTCCAATGTCCGCTTTAAAGGCGATGACGGGAAAGCAGaa > 1:275894/1‑90 (MQ=255) gcgcGTCACCGACATCGAACCGGGTCTGGGGGGGGGTACCGG‑GGTTTCCAATGTCCGCTTTAAAGGCGGTGACGGTAAAGCAGAAAAAAc > 1:343561/1‑90 (MQ=255) gcgcGTCACCGACATCGAACCGGGGCTGGTGGGGGGTACCGG‑GGTTTTCAATGTCCGCTTTAAAGGGGGTGGCGGGAAAGCAGAAAAAAc > 1:278960/1‑90 (MQ=255) tCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGA‑GTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATc < 2:275894/90‑1 (MQ=255) gggTCTGGTGGGTGGTACCGA‑GTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCAtt > 2:554110/1‑90 (MQ=255) tgggtggTACCGA‑GTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCag < 2:422882/90‑1 (MQ=255) | GTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGA‑GTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAG > NZ_CP009273/1622237‑1622401 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |