| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,567 | 0 | T | G | 56.2% | 9.1 / 14.6 | 16 | G81G (GGT→GGG) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (9/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCG‑AAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAC > NZ_CP009273/2089479‑2089651 | tCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTg < 1:370705/90‑1 (MQ=255) cTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTa < 2:135071/90‑1 (MQ=255) tCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAg < 1:259846/90‑1 (MQ=255) ggCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGGTTCTTGaa > 1:75529/1‑90 (MQ=255) gggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGGGTCTGGAAGGTGCCCCGCAAAATTTTTTct > 1:476785/1‑89 (MQ=255) ggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAATCTCTGGGAGATGGCGCG‑AAAAATTTTTTCtt > 1:173913/1‑90 (MQ=255) aTCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTTTGTGGCGGTTGGGAATAAATGTCCGCAACAAGCCCCG‑CAAATtcttttcttttg > 1:216834/1‑88 (MQ=255) cgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGAAGCCGCG‑AAAAATTTTTTCTTTTGGGCGGa > 2:294845/1‑90 (MQ=255) cgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGCCGCG‑AAAATTCTTTTCTTTGGCGCggg > 2:135606/1‑89 (MQ=255) tGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGCCGCG‑AAAATTCTTTTCTTTTGCGCGGGTa > 2:242322/1‑90 (MQ=255) gACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCG‑AAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAAc < 1:484068/90‑1 (MQ=255) attacGTTTTGTGTGGCGGGGGGGGTTAGGTCTCTGGAAGATGCCGCG‑AAAATTTTTTTCTTTGGCGCGGGTAAAAATTCCATCAActct > 2:260465/5‑90 (MQ=255) ttCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCCCG‑AAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACtctc > 2:51710/1‑90 (MQ=255) ttCCGTTTTGTGTGGCGGGGGGGGGTAAGTCTCTGGGAGAAGGCGCGAAAAATTTTTTCTTTTGGGGGGGTAAAAATTTCC‑TCAACactc > 1:167844/1‑90 (MQ=255) tttGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCG‑AAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTgcgc < 2:75529/90‑1 (MQ=255) gCGGGGGGGGTTAAGTCTCTGGAAGATGCCGCG‑AAAAATTTTTTCTTTTGGGCGGGTAAAATTTTCATCAAATCTCCTGCGCTGGCAGAc > 1:171608/1‑90 (MQ=255) | TCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCG‑AAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAC > NZ_CP009273/2089479‑2089651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |