| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,958 | 0 | G | A | 63.2% | ‑5.5 / 12.0 | 19 | L57L (CTG→TTG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (7/0); new base A (0/12); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.98e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058874‑4059038 | tGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGCAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGa > 2:318002/1‑90 (MQ=255) ggCCAAATTTTTTCCCGGGAGTCGAAAAAAAACCGGGGGAGCATATCGGAAAACGCGGAAAAAAACTTCGCCACAAAAAAAATAACCCCg < 1:32090/90‑1 (MQ=255) ttttttCCGCGAGTCGAGAAAAAATCCGGTGAGCATATCGGAAACCGCGGAAAAAAACTCCCCCCCCAAAAAGATAACCCCGCCATAGTa < 2:46088/89‑1 (MQ=255) gggagtcgaaaAAAAACCGGGGGAGCATATGGGAAAACGCGGAAAAAACCTCCCCCACAAAAAAAATAATCCCGCCATAGTAAGCAGGCa < 1:61814/88‑1 (MQ=255) gcgGGTCGAGTAAAAACCGGGTGAGCATATCGGTAAACGGGTTAAAAAACTCCCCCCCCAAAAAGAAAATCCCGCCATAGTAAGCAGGCa < 2:336393/90‑1 (MQ=255) gcgAGTCGAAAAAAAACCGGGGGAGAATATGGGAAAACGCGAAAAAAACCTCCCCCCCAAAAAAAATAACCCCGCCATAGTAAGCAGGCa < 1:467181/90‑1 (MQ=255) aaaaCCGGGTGAGCATATCGAAAAACGGGGAAAAAACCTTCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCAt < 1:107345/90‑1 (MQ=255) aaTCCGGGGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGGAAGCAGGCATCCCTAAGTCATCg > 1:274938/1‑90 (MQ=38) cGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtat > 1:465119/1‑90 (MQ=255) tCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg > 1:34428/1‑90 (MQ=255) tCGGAAACCGCGAAAAAAAACTTCGCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg < 2:418043/90‑1 (MQ=255) gaaaaCGCGTAAAAAAACTCCCCCCCCAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGgaga < 2:507743/88‑1 (MQ=255) gTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGgaga > 1:6021/1‑90 (MQ=255) gTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGgaga > 1:32843/1‑90 (MQ=255) accgcgGAAAAAACCTCCCCCACAAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATAc < 2:377079/88‑1 (MQ=255) accgcgGAAAAAAACTCCCCCCCAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATAc < 2:115171/88‑1 (MQ=255) ggaaaaaacCTCCCCCCCCAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGt < 2:464238/87‑1 (MQ=255) tcccccacaaAAAAAATACTCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag < 2:465119/86‑1 (MQ=255) tCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga > 1:339973/1‑90 (MQ=255) | TGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058874‑4059038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |