Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,682,423	G→C	A71G (GCC→GGC)	fumB ←	class I fumarate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,682,423	0	G	C	100.0%	33.1 / NA	12	A71G (GCC→GGC)	fumB	class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (7/5);  total (7/5)

TTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGC  >  NZ_CP009273/1682338‑1682499                                                                                      |                                                                             tttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCAc                                                                          >  2:482071/1‑90 (MQ=255)           aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTgg                                                                <  1:589659/90‑1 (MQ=255)           aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTgg                                                                <  1:81227/90‑1 (MQ=255)                ggAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTgcgc                                                           <  2:141078/90‑1 (MQ=255)                     tACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGAc                                                      >  2:249662/1‑90 (MQ=255)                      aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACg                                                     <  1:482071/90‑1 (MQ=255)                                                tATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACg                           >  1:456444/1‑90 (MQ=255)                                                     ttttCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTgg                      >  1:571152/1‑90 (MQ=255)                                                     ttttCGCTGGCCTCCGGGTCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTgg                      >  2:350874/1‑90 (MQ=255)                                                                       tCACGCAGAATGTCGCCTACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAAc    <  1:43813/90‑1 (MQ=255)                                                                       tCACGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACCCATCATGAAACGCCTGGCGCGCCAACAGAGTTAAc    >  2:328183/1‑90 (MQ=255)                                                                         aCGCAGAATGTCGCCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGc  >  2:536985/1‑90 (MQ=255)                                                                                      |                                                                             TTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGC  >  NZ_CP009273/1682338‑1682499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑