| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 181,707 | C→G | G243A (GGC→GCC) | dapD ← | 2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 181,707 | 0 | C | G | 95.5% | 66.3 / ‑3.1 | 22 | G243A (GGC→GCC) | dapD | 2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (11/10); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.81e-01 | |||||||||||
GTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGTAGTGGATTTCGCCGGTTTCACGGTCG > NZ_CP009273/181620‑181790 | gTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGGCa < 2:475212/90‑1 (MQ=255) gcagTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGGCATCTTTTg > 1:166992/1‑90 (MQ=255) ttCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGGCATCTTTTGACgg < 2:197978/90‑1 (MQ=255) gCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGGCATCTTTTGACGGCAGATTAcc > 2:140914/1‑90 (MQ=255) ttGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCCCAGTAGAGGCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAaaca < 1:496993/90‑1 (MQ=255) tCTTAACGATAACCGCACAGTAGAGGCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGAc < 2:410361/90‑1 (MQ=255) cGATAACCGCACAGTAGAGGCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGTAGt < 2:539945/90‑1 (MQ=255) acaGTAGAGGCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGAc < 2:232198/74‑1 (MQ=255) acaGTAGAGGCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGAc > 1:232198/1‑74 (MQ=255) gTAGAGGCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGTAGTGGATTTCGCCGGt > 1:251012/1‑90 (MQ=255) gagGCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGAc < 1:316315/68‑1 (MQ=255) gagGCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGAc > 2:316315/1‑68 (MQ=255) ggCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGTAGTGGATTTCGCCGGTTTCAc > 1:50209/1‑90 (MQ=255) ggCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGTAGTGGATTTCGCCGGTTTCAc < 1:554904/90‑1 (MQ=255) ggCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGTAGTGGATTTCGCCGGTTTCAc > 1:324087/1‑90 (MQ=255) ggCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGTAGTGGATTTCGCCGGTTTCAc > 2:14641/1‑90 (MQ=255) ggCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGTAGTGGATTTCGCCGGTTTCAc < 2:300331/90‑1 (MQ=255) ggCTGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGTAGTGGATTTCGCCGGTTTCAc < 2:509436/90‑1 (MQ=255) tGTATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGTAGTGGATTTCGCCGGTTTCACGGt > 2:506588/1‑90 (MQ=255) tATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGTAGTGGATTTCGCCGGTTTCACGGTCg > 2:12053/1‑90 (MQ=255) tATTTGGCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGGAGTGGATTTCGCCGGTTTCACGGTCg > 1:299686/1‑90 (MQ=255) tATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACCGCAGACCCCGCCGGAACGCGACCGTAGTGGATTTCGCCGGTTTCACGGTCg > 1:468282/1‑90 (MQ=255) | GTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACGCGACCGTAGTGGATTTCGCCGGTTTCACGGTCG > NZ_CP009273/181620‑181790 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |