Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,003,480	T→C	F60S (TTC→TCC)	yedK →	SOS response‑associated peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,003,480	0	T	C	100.0%	29.0 / NA	11	F60S (TTC→TCC)	yedK	SOS response‑associated peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/5);  total (6/5)

AACCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGT  >  NZ_CP009273/2003393‑2003556                                                                                        |                                                                             aaCCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCt                                                                            <  2:425601/90‑1 (MQ=255)     cATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTgggg                                                                        >  1:439843/1‑90 (MQ=255)       ttGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGat                                                                      >  2:477341/1‑90 (MQ=255)         ggCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGatat                                                                    <  2:114469/90‑1 (MQ=255)             gATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTc                                                                >  1:167821/1‑90 (MQ=255)                     gTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGAtgg                                                        <  2:285435/90‑1 (MQ=255)                        gcgcCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGAtggtgg                                                     >  1:193826/1‑90 (MQ=255)                                                 tCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCGGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGccc                            <  1:547006/90‑1 (MQ=255)                                                      gAACGTGATGAACCCCTTCATCTGGATCCGGTTTCCGGGGGATATGCCCCCGGATGGGGGGATAAACCGCCGCTGATTAACGCCCGCGTa                       <  1:313998/90‑1 (MQ=255)                                                         cGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGaa                    >  2:208577/1‑90 (MQ=255)                                                                           cTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGt  >  1:525594/1‑90 (MQ=255)                                                                                        |                                                                             AACCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGT  >  NZ_CP009273/2003393‑2003556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑