Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,131,004	A→G	intergenic (‑280/‑379)	wza ← / → yegH	polysaccharide export protein Wza/TerC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,131,004	0	A	G	100.0%	74.6 / NA	24	intergenic (‑280/‑379)	wza/yegH	polysaccharide export protein Wza/TerC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/17);  total (7/17)

TTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAA  >  NZ_CP009273/2130921‑2131085                                                                                    |                                                                                  tttttAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTaa                                                                             <  2:585576/90‑1 (MQ=255)      aGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTcc                                                                        <  2:483143/90‑1 (MQ=255)            cAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAatat                                                                  <  1:196575/90‑1 (MQ=255)                acacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAgg                                                              >  2:544153/1‑90 (MQ=255)                 cgcTGATTTAGTTTAATGTTTCCCTCCCTCCCACCATTCAGTCGTTGATGAGAAAGGGTTATTCCGGGAATGAACCGCCGAATATAAGGt                                                             <  1:97065/88‑1 (MQ=255)                 cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                             <  1:57975/90‑1 (MQ=255)                 cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                             <  2:561913/90‑1 (MQ=255)                 cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                             <  1:355350/90‑1 (MQ=255)                 cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                             <  1:295938/90‑1 (MQ=255)                 cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                             <  1:258885/90‑1 (MQ=255)                  acTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTg                                                            <  2:163731/90‑1 (MQ=255)                     gATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACa                                                         >  2:5780/1‑90 (MQ=255)                     gATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACa                                                         >  1:581241/1‑90 (MQ=255)                     gATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACa                                                         >  2:434766/1‑90 (MQ=255)                                aTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAAtt                                              <  1:314716/90‑1 (MQ=255)                                             tctcAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTGATTGAATATTGGCttt                                 >  2:247792/1‑90 (MQ=255)                                             tctcAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCttt                                 <  1:18905/90‑1 (MQ=255)                                               tcAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTcc                               <  2:59166/90‑1 (MQ=255)                                                       cAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTcc                                                                        <  2:498073/41‑1 (MQ=255)                                                       cAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTcc                                                                        >  1:498073/1‑41 (MQ=255)                                                               gatgaGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGaa               <  2:276079/90‑1 (MQ=255)                                                                atgaGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAg              <  1:196398/90‑1 (MQ=255)                                                                           ttattaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTa   <  2:503994/90‑1 (MQ=255)                                                                            tattaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTaa  >  1:202553/1‑90 (MQ=255)                                                                                    |                                                                                  TTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAA  >  NZ_CP009273/2130921‑2131085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑