Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,176,805	T→G	D109A (GAC→GCC)	yegX ←	glycoside hydrolase family 25 protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,176,805	0	T	G	100.0%	55.5 / NA	19	D109A (GAC→GCC)	yegX	glycoside hydrolase family 25 protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/10);  total (9/10)

GGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCATTTTTGCCACCCGCTGCC  >  NZ_CP009273/2176732‑2176889                                                                          |                                                                                     ggAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGcc                                                                      <  2:8567/90‑1 (MQ=255)  gagaAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCt                                                                     <  1:568984/90‑1 (MQ=255)       ataatGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGt                                                                <  2:78576/90‑1 (MQ=255)               aCGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGGAAAATAGGGGGCCACCAACTTATCGCCTTCCGTCGCCTtaa                                                        >  1:552819/1‑90 (MQ=255)               aCGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGCCTtaa                                                        >  2:339518/1‑90 (MQ=255)               aCGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGAGGGGCACCAGCTTTTCGCCTTCCGTCGCCTtaa                                                        >  2:364277/1‑90 (MQ=255)                  ccccACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGCCTtaataa                                                     >  2:428603/1‑90 (MQ=255)                  ccccACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGCCTtaataa                                                     >  2:39847/1‑90 (MQ=255)                  ccccACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGCCTtaataa                                                     <  2:175351/90‑1 (MQ=255)                      aCGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGc                                                 >  1:72801/1‑90 (MQ=255)                                     ttCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGcc                                                            <  1:212028/64‑1 (MQ=255)                                     ttCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGcc                                                            >  2:212028/1‑64 (MQ=255)                                     ttCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGAt                                  <  1:515358/90‑1 (MQ=255)                                     ttCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGAt                                  <  1:428603/90‑1 (MQ=255)                                          ggCTTAGTTGCCAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCAt                             <  1:272614/90‑1 (MQ=255)                                                    ccAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCAt                   <  1:329009/90‑1 (MQ=255)                                                     cAGTTACGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCAtt                  >  2:566636/1‑90 (MQ=255)                                                          aCGCGAAAAATAGGGGGCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCATTTTTGc             >  1:521752/1‑90 (MQ=255)                                                                     aGGGGGCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCATTTTTGCCACCCGCTGcc  <  1:544418/90‑1 (MQ=255)                                                                          |                                                                                     GGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCATTTTTGCCACCCGCTGCC  >  NZ_CP009273/2176732‑2176889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑