Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,202,478	T→G	Y345D (TAT→GAT)	yehP →	VWA domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,202,478	0	T	G	100.0%	26.7 / NA	10	Y345D (TAT→GAT)	yehP	VWA domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/2);  total (8/2)

TACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCT  >  NZ_CP009273/2202392‑2202560                                                                                       |                                                                                   tACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCGATg                                                                                 >  1:195194/1‑90 (MQ=255)                        tgtgtCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCGATGACCGCGATACGGCCCAGGCGCTg                                                          >  1:337693/1‑90 (MQ=255)                           gtCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCGATGACCGCGATGCGGCCCAGGCGCTgggt                                                       >  1:572301/1‑88 (MQ=255)                                                       tGGCAGCGCTCGATAGCACCGCAACACCTTGCGATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGa                           >  1:213839/1‑90 (MQ=255)                                                                  gATAGCACCGCAACACCTTGCGATGACCGCGATACGGCCCAGGCGCTGGTCAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAg                >  1:531451/1‑90 (MQ=255)                                                                      gCACCGCAACACCTTGCGATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTgg            >  1:433213/1‑90 (MQ=255)                                                                         ccGCAACACCTTGCGATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGgc                                              >  1:189697/1‑53 (MQ=255)                                                                         ccGCAACACCTTGCGATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGgc                                              <  2:189697/53‑1 (MQ=255)                                                                           gCAACACCTTGCGATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcatca       >  1:117483/1‑90 (MQ=255)                                                                                acCTTGCGATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCt  <  2:45256/90‑1 (MQ=255)                                                                                       |                                                                                   TACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCT  >  NZ_CP009273/2202392‑2202560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑