Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,410,868	A→T	G103G (GGA→GGT)	yfcC →	putative basic amino acid antiporter YfcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,410,868	0	A	T	100.0%	99.9 / NA	29	G103G (GGA→GGT)	yfcC	putative basic amino acid antiporter YfcC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/13);  total (16/13)

ACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCA  >  NZ_CP009273/2410781‑2410953                                                                                        |                                                                                      aCCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTc                                                                                     >  2:184454/1‑90 (MQ=255)               gcgTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAg                                                                       >  2:393822/1‑90 (MQ=255)               gcgTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAg                                                                       >  2:226311/1‑90 (MQ=255)                 gTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGcc                                                                     >  1:94888/1‑90 (MQ=255)                    cAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGtt                                                                  >  2:463814/1‑90 (MQ=255)                        tGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGca                                                              <  2:73092/90‑1 (MQ=255)                         gTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGcat                                                             >  1:289193/1‑90 (MQ=255)                         gTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGcat                                                             >  2:237028/1‑90 (MQ=255)                         gTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGcat                                                             >  1:50018/1‑90 (MQ=255)                                acgGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATg                                                      >  1:21390/1‑90 (MQ=255)                                acgGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATg                                                      >  2:272031/1‑90 (MQ=255)                                  gggCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGtt                                                    <  1:581744/90‑1 (MQ=255)                                   ggCGATGAACGCCCGGGCCGGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGtgt                                                   >  1:69133/1‑88 (MQ=255)                                             gCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGa                                         >  1:311081/1‑90 (MQ=255)                                                    ccTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTggcgg                                  <  1:237028/90‑1 (MQ=255)                                                    ccTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTggcgg                                  <  1:393822/90‑1 (MQ=255)                                                            aCTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGttt                           <  1:536353/89‑1 (MQ=255)                                                            aCTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGttt                           >  2:536353/1‑89 (MQ=255)                                                                cccGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCAt                      <  1:463814/90‑1 (MQ=255)                                                                  cGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATG‑TTATGCTGGTGATTGGCGGCGCGTTTGGCATtgt                   >  2:279002/1‑90 (MQ=255)                                                                    tttGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATtgtg                  <  2:80871/90‑1 (MQ=255)                                                                       gAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATg               <  1:272031/90‑1 (MQ=255)                                                                       gAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATg               <  1:24762/90‑1 (MQ=255)                                                                       gAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATg               <  1:205400/90‑1 (MQ=255)                                                                          ggATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATG‑TTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTa           <  1:279002/90‑1 (MQ=255)                                                                               aaCCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAgg       >  1:393236/1‑90 (MQ=255)                                                                                    cAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGgcggcg                                >  2:495976/1‑60 (MQ=255)                                                                                    cAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGgcggcg                                <  1:495976/60‑1 (MQ=255)                                                                                    cAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCa  <  2:180461/90‑1 (MQ=255)                                                                                        |                                                                                      ACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCA  >  NZ_CP009273/2410781‑2410953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑