Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	231,042	A→C	G76G (GGT→GGG)	gloB ←	hydroxyacylglutathione hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	231,042	0	A	C	100.0%	34.9 / NA	12	G76G (GGT→GGG)	gloB	hydroxyacylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/6);  total (6/6)

TACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGAGAAAT  >  NZ_CP009273/230958‑231123                                                                                     |                                                                                  tACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATa                                                                              >  2:560293/1‑90 (MQ=255)  acacTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATac                                                                             <  2:175121/90‑1 (MQ=255)                        cGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACtttt                                                       <  1:370976/90‑1 (MQ=255)                                             tGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTAcgccgc                                  <  1:266586/90‑1 (MQ=255)                                             tGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTAcgccgc                                  <  2:574728/90‑1 (MQ=255)                                              gACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACgccgcc                                 <  2:490778/90‑1 (MQ=255)                                                   ccTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGt                            >  1:119628/1‑90 (MQ=255)                                                          tgttCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCgtg                     >  1:504854/1‑90 (MQ=255)                                                             tCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCgtggtg                  >  2:57534/1‑90 (MQ=255)                                                                          gtCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATggtgggtg        <  1:89112/87‑1 (MQ=255)                                                                          gtCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATggtgggtg        >  2:89112/1‑87 (MQ=255)                                                                             tctTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGGTATGGTGGGTGAGAAat  >  1:477672/1‑90 (MQ=255)                                                                                     |                                                                                  TACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGAGAAAT  >  NZ_CP009273/230958‑231123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑