Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,678,723	A→C	G48G (GGT→GGG)	glyA ←	serine hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,678,723	0	A	C	100.0%	63.3 / NA	21	G48G (GGT→GGG)	glyA	serine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/10);  total (11/10)

AGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTG  >  NZ_CP009273/2678637‑2678806                                                                                       |                                                                                    aGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAcccc                                                                                  >  1:52729/1‑90 (MQ=255)             atacCAACATCCCCCCACCCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCAtt                                                                      <  2:529942/90‑1 (MQ=255)               atCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTAc                                                                    >  1:125171/1‑90 (MQ=255)               atCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTAc                                                                    >  1:220855/1‑90 (MQ=255)                      tACTCGCAATCGCCGTAGTAGCGTTTGCCCGGATAGCCTTCAGCTGGTTTGTTGGTCAGCTTAGACCCCTGCGCCTGCATTACGCGCggg                                                             >  1:468843/1‑90 (MQ=255)                           gCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTggg                                                        >  2:544772/1‑89 (MQ=255)                            cacccgccgTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGgtg                                                       <  1:126913/87‑1 (MQ=255)                               ccgccgTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAg                                                    <  1:296749/90‑1 (MQ=255)                                            gTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAgg                                          >  2:517225/1‑87 (MQ=255)                                            gTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAgg                                          <  1:517225/87‑1 (MQ=255)                                            gTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGa                                       >  1:1396/1‑90 (MQ=255)                                            gTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGa                                       >  1:560924/1‑90 (MQ=255)                                             tttGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGAt                                      <  1:148509/90‑1 (MQ=255)                                              ttGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATc                                     >  1:96255/1‑90 (MQ=255)                                                                gCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTcc                   <  2:387813/90‑1 (MQ=255)                                                                      ttgttgGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGt             >  1:142740/1‑90 (MQ=255)                                                                          tgGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACtt         >  1:501061/1‑90 (MQ=255)                                                                               aGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTcc    <  2:517325/90‑1 (MQ=255)                                                                               aGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTcc    <  1:12665/90‑1 (MQ=255)                                                                                gCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTGGTTTTCGGAGGCGGTCAGTTCGATGTGCTCTTCCTGAGGTACTGTTTCCt   >  1:469126/1‑90 (MQ=255)                                                                                gCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCt   <  1:262289/90‑1 (MQ=255)                                                                                 cTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTg  <  2:122191/90‑1 (MQ=255)                                                                                       |                                                                                    AGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTTTTTCCTG  >  NZ_CP009273/2678637‑2678806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑