Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	297,509	G→C	G30G (GGC→GGG)	paoB ←	aldehyde oxidoreductase FAD‑binding subunit PaoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	297,509	0	G	C	100.0%	74.5 / NA	24	G30G (GGC→GGG)	paoB	aldehyde oxidoreductase FAD‑binding subunit PaoB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (12/12);  total (12/12)

ATCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTT  >  NZ_CP009273/297428‑297595                                                                                  |                                                                                       aTCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGAt                                                                                >  2:513316/1‑90 (MQ=255) aTCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGAt                                                                                >  2:316497/1‑90 (MQ=255) aTCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGGGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGAt                                                                                >  2:423709/1‑90 (MQ=255)      gCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAAtt                                                                           >  2:501411/1‑90 (MQ=255)                gTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCggg                                                                 >  2:188137/1‑90 (MQ=255)                        cGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCt                                                         <  1:226061/90‑1 (MQ=255)                          aTAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGa                                                       >  2:118087/1‑90 (MQ=255)                                 gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTaa                                                >  2:161151/1‑90 (MQ=255)                                 gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTaa                                                >  2:300360/1‑90 (MQ=255)                                         gTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTgccgcc                                        <  2:144422/90‑1 (MQ=255)                                              aaTTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGc                                   <  1:161151/90‑1 (MQ=255)                                                tttCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTg                                 <  1:316497/90‑1 (MQ=255)                                                        ttCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTc                         <  1:274142/90‑1 (MQ=255)                                                            tcaGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTc                     <  2:242963/90‑1 (MQ=255)                                                            tcaGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTc                     <  2:484544/90‑1 (MQ=255)                                                                gttcagcagATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTc                 <  1:477422/87‑1 (MQ=255)                                                                gTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTc                 >  1:393907/1‑90 (MQ=255)                                                                  ccAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCAt               <  2:65897/90‑1 (MQ=255)                                                                   cagcagATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCCCTCGTTCATa              <  2:361141/90‑1 (MQ=255)                                                                        gATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTaa         >  1:81019/1‑90 (MQ=255)                                                                        gATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTaa         <  1:423709/90‑1 (MQ=255)                                                                          ttGGTCCCCCCCGCGACAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAAc       >  1:357155/1‑90 (MQ=255)                                                                             gTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTa                                                 <  2:453435/45‑1 (MQ=255)                                                                             gTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTa                                                 >  1:453435/1‑45 (MQ=255)                                                                               cccccccgCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCtt  >  2:394132/1‑90 (MQ=255)                                                                               cccccccgCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCtt  >  2:282810/1‑90 (MQ=255)                                                                                  |                                                                                       ATCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTT  >  NZ_CP009273/297428‑297595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑