Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,475,701	T→C	S352P (TCG→CCG)	yheS →	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,475,701	0	T	C	100.0%	24.3 / NA	9	S352P (TCG→CCG)	yheS	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/6);  total (3/6)

CGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTAC  >  NZ_CP009273/3475636‑3475784                                                                  |                                                                                    cgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAACCGACATTAATCAAACTGTTAGCCg                                                             >  1:559440/1‑90 (MQ=255)             aCCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAACCGACATTAATCAAACTGTTAGCCGGTGAACTTgcgc                                                 <  2:166632/90‑1 (MQ=255)                  gTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAACCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTc                                            <  2:11335/90‑1 (MQ=255)                       cGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAACCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCgg                                       >  1:430705/1‑90 (MQ=255)                              cgTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAACCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAAtt                                <  1:522623/90‑1 (MQ=255)                                   tGGTCTGTTAGGCCGCAATGGCGCGGGTAAACCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCt                           <  1:421010/90‑1 (MQ=255)                                   tGGTCTGTTAGGCCGCAATGGCGCGGGTAAACCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCt                           <  2:148213/90‑1 (MQ=255)                                         gTTAGGCCGCAATGGCGCGGGTAAACCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGaa                     >  1:582151/1‑90 (MQ=255)                                                            ggTAAACCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTAc  <  2:338022/90‑1 (MQ=255)                                                                  |                                                                                    CGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTAC  >  NZ_CP009273/3475636‑3475784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑