Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,785,687	G→C	G75G (GGC→GGG)	kbl ←	glycine C‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,785,687	0	G	C	100.0%	71.2 / NA	23	G75G (GGC→GGG)	kbl	glycine C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (18/5);  total (18/5)

CCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGG  >  NZ_CP009273/3785605‑3785766                                                                                   |                                                                                ccATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAAc                                                                          <  1:470792/90‑1 (MQ=255)        cAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTgaga                                                                   >  1:197132/1‑90 (MQ=255)               gccgccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCAtt                                                            >  2:485627/1‑90 (MQ=255)                ccgccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTc                                                           >  1:461727/1‑90 (MQ=255)                          ttGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTgc                                                 <  2:217861/90‑1 (MQ=255)                                aaGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCcgccgc                                           >  1:99315/1‑90 (MQ=255)                                      tttATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatcag                                     >  1:78853/1‑90 (MQ=255)                                             cTGTCCTGAGTGCCGCAAATAAAACGCCCCGAAGCCACCCCGAACCCGTGAGAACCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGga                              <  1:337475/90‑1 (MQ=255)                                               gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                            >  1:465542/1‑90 (MQ=255)                                               gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                            >  1:125778/1‑90 (MQ=255)                                               gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                            >  1:294544/1‑90 (MQ=255)                                               gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                            >  2:252647/1‑90 (MQ=255)                                               gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                            >  2:87355/1‑90 (MQ=255)                                               gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCACCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                            >  1:9563/1‑90 (MQ=255)                                               gTCCTGAGGGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCCCAATCAGATCAGgatg                            >  1:402864/1‑90 (MQ=255)                                                         ccGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGc                  >  1:296159/1‑90 (MQ=255)                                                         ccGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGc                  >  1:228315/1‑90 (MQ=255)                                                           gCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc                >  2:143260/1‑90 (MQ=255)                                                           gCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc                <  2:99315/90‑1 (MQ=255)                                                            cAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCg               >  2:335446/1‑90 (MQ=255)                                                                  aaccGCACCGAAGCCATCCCAAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatc                                       <  1:348078/57‑1 (MQ=255)                                                                  aaaCGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatc                                       >  2:348078/1‑60 (MQ=255)                                                                         ccGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTgg  >  2:577595/1‑90 (MQ=255)                                                                                   |                                                                                CCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGG  >  NZ_CP009273/3785605‑3785766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑