| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,903 | T→A | D27E (GAT→GAA) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,903 | 0 | T | A | 95.5% | 63.9 / ‑4.2 | 22 | D27E (GAT→GAA) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (8/13); total (9/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01 | |||||||||||
AATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTC > NZ_CP009273/49822‑49976 | aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTg < 2:13537/90‑1 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTaa > 1:34488/1‑90 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa < 2:34488/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa < 1:516926/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa < 1:563254/90‑1 (MQ=255) aTTGCGGCGTTAGCGGTAGCTCGCGTTATCGGCATGGAAAACGCCACGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac < 2:532342/90‑1 (MQ=255) aTTGCGGCGTTAGCGGGAGATCGCGTTATCGGCATGGGAAACCCCAATCCGCGGAAGCCCCCCGCCGAACTCCCCTTGGTTAAACggcaa > 1:382916/1‑86 (MQ=255) ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTtaaat > 2:146638/1‑90 (MQ=255) ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGGTTAAACGCAACACCTtaaat > 1:377135/1‑90 (MQ=255) tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTtaaata < 1:416148/90‑1 (MQ=255) aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTtaaataa < 1:176479/90‑1 (MQ=255) gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTg > 2:566787/1‑90 (MQ=255) gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTg > 1:510300/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCCGCCTGCCGAACTCGCCTGGGTTAAACGCAACACCTTAAATAAACCCGTGATTa > 1:533818/1‑90 (MQ=255) taccGGCATGGAAACCGCCATGCCGTGGACCCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg < 2:274902/87‑1 (MQ=255) tCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgcc < 2:235893/90‑1 (MQ=255) cATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa < 2:469770/90‑1 (MQ=255) aaaaCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt < 1:184666/90‑1 (MQ=255) aTGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca > 1:261144/1‑90 (MQ=255) ggAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc < 1:172553/90‑1 (MQ=255) tgcctgccGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc > 2:128484/1‑84 (MQ=255) tgcctgccGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc < 1:128484/84‑1 (MQ=255) | AATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTC > NZ_CP009273/49822‑49976 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |