Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,527,151	T→C	Q87Q (CAA→CAG)	nanS ←	9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,527,151	0	T	C	100.0%	101.2 / NA	31	Q87Q (CAA→CAG)	nanS	9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/15);  total (16/15)

ATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTTTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTAA  >  NZ_CP009273/4527068‑4527236                                                                                    |                                                                                      aTAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCtgatga                                                                                 >  2:125806/1‑90 (MQ=255) aTAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCtgatga                                                                                 >  2:7343/1‑90 (MQ=255) aTAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCtgatga                                                                                 >  1:108643/1‑90 (MQ=255)     gAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCg                                                                             <  2:108643/90‑1 (MQ=255)       aTCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGtt                                                                           <  2:404859/90‑1 (MQ=255)        tCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTg                                                                          >  2:107189/1‑90 (MQ=255)               aTTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAgg                                                                   >  1:297043/1‑90 (MQ=255)                  aTCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                <  2:391382/90‑1 (MQ=255)                  aTCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                <  2:394594/90‑1 (MQ=255)                       gAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGAt                                                           <  2:347198/90‑1 (MQ=255)                           gAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAAcc                                                       <  1:319042/90‑1 (MQ=255)                                gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  1:432523/90‑1 (MQ=255)                                gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  >  1:141110/1‑90 (MQ=255)                                 cAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCat                                                 >  2:140300/1‑90 (MQ=255)                                          ccGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAAc                                        >  2:468854/1‑90 (MQ=255)                                          ccGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAAc                                        <  1:426193/90‑1 (MQ=255)                                            gTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACAt                                      >  2:589059/1‑90 (MQ=255)                                                 atatGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCgtgt                                 <  2:411624/90‑1 (MQ=255)                                                        gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  >  1:333873/1‑66 (MQ=255)                                                        gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  2:333873/66‑1 (MQ=255)                                                        gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCt                                            >  2:550577/1‑72 (MQ=255)                                                        gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCt                                            <  1:550577/72‑1 (MQ=255)                                                             tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc                     <  1:125806/90‑1 (MQ=255)                                                                ccAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGt                  <  2:297043/90‑1 (MQ=255)                                                                      gTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGaata            >  1:463223/1‑90 (MQ=255)                                                                        gCCGTACTGTGTCTGGTGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGaa                                         <  2:455683/59‑1 (MQ=255)                                                                        gCCGTACTGTGTCTGGTGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGaa                                         >  1:455683/1‑59 (MQ=255)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  <  2:288611/90‑1 (MQ=255)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  1:524923/1‑90 (MQ=255)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGGGGAATAATGTCGTTaa  >  1:433355/1‑90 (MQ=255)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCACATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  2:360622/1‑90 (MQ=255)                                                                                    |                                                                                      ATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTTTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTAA  >  NZ_CP009273/4527068‑4527236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑