Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	568,752	A→G	G71G (GGA→GGG)	ybcO →	DUF1364 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	568,752	0	A	G	100.0%	79.4 / NA	25	G71G (GGA→GGG)	ybcO	DUF1364 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/18);  total (7/18)

TACGAAACCGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGATATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATACCTAC  >  NZ_CP009273/568665‑568838                                                                                        |                                                                                       tccGAACCCGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGgta                                                                                      <  1:10584/88‑1 (MQ=255)           ccAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGaa                                                                            <  1:168138/90‑1 (MQ=255)                  tGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATgcgcgc                                                                     >  2:528372/1‑90 (MQ=255)                      tGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGa                                                                 <  2:352286/90‑1 (MQ=255)                        ccaccaTTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAg                                                               <  2:30247/90‑1 (MQ=255)                                gCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCg                                                       <  1:379107/90‑1 (MQ=255)                                     ttCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAac                                                  <  2:388438/90‑1 (MQ=255)                                     ttCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAac                                                  >  2:3063/1‑90 (MQ=255)                                         gcctgccACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAg                                              >  1:110440/1‑90 (MQ=255)                                         gcctgccACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAg                                              <  2:442490/90‑1 (MQ=255)                                            tgccACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAg                                                               <  2:493995/70‑1 (MQ=255)                                            tgccACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAg                                                               >  1:493995/1‑70 (MQ=255)                                               cACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATc                                        <  1:25180/90‑1 (MQ=255)                                                           gACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAagag                            <  2:218614/90‑1 (MQ=255)                                                            aCCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAgg                           <  1:528372/90‑1 (MQ=255)                                                             ccgccgCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAggg                          <  2:274545/90‑1 (MQ=255)                                                                          tttGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCga                                                      <  2:149780/49‑1 (MQ=255)                                                                          tttGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCga                                                      >  1:50739/1‑49 (MQ=255)                                                                          tttGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCga                                                      >  1:149780/1‑49 (MQ=255)                                                                          tttGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCga                                                      <  2:50739/49‑1 (MQ=255)                                                                           ttgttgACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGt            <  1:189804/90‑1 (MQ=255)                                                                               tgACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAAt        <  1:173582/90‑1 (MQ=255)                                                                                gACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATa       <  2:389212/90‑1 (MQ=255)                                                                                 aCGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATAc      >  2:239616/1‑90 (MQ=255)                                                                                     tGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATACCTAc  <  2:49110/90‑1 (MQ=255)                                                                                     tGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATACCTAc  <  1:107123/90‑1 (MQ=255)                                                                                        |                                                                                       TACGAAACCGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGATATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATACCTAC  >  NZ_CP009273/568665‑568838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑