Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	874,237	C→T	L14F (CTT→TTT)	yliI →	aldose sugar dehydrogenase YliI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	874,237	0	C	T	100.0%	83.0 / NA	22	L14F (CTT→TTT)	yliI	aldose sugar dehydrogenase YliI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/10);  total (12/10)

ATCTCCTCCACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGGCCTTT  >  NZ_CP009273/874150‑874323                                                                                        |                                                                                       aTCTCCTCCACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGtttt                                                                                      <  1:555136/90‑1 (MQ=255)      ctccACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTttcttc                                                                                 >  1:543494/1‑90 (MQ=255)           cTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGctc                                                                            >  2:155743/1‑90 (MQ=255)             gACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGctctc                                                                          >  1:176170/1‑90 (MQ=255)                            cAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGACGGCTCCTGCa                                                           <  1:258270/90‑1 (MQ=255)                             agagTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCaa                                                          <  2:176170/90‑1 (MQ=255)                              gagTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCaa                                                          >  1:11288/1‑89 (MQ=255)                              gagTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCaa                                                          <  2:11288/89‑1 (MQ=255)                              gagTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAAc                                                         >  2:567269/1‑90 (MQ=255)                              gagTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAAc                                                         >  2:433047/1‑90 (MQ=255)                              gagTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAAc                                                         >  2:121833/1‑90 (MQ=255)                                     tCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAAt                                                  <  2:335963/90‑1 (MQ=255)                                        ggAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTc                                               >  2:110636/1‑90 (MQ=255)                                        ggAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTc                                               >  1:582373/1‑90 (MQ=255)                                         gAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCg                                              >  1:496534/1‑90 (MQ=255)                                                  tGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGc                                     <  2:56260/90‑1 (MQ=255)                                                       cGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGAc                                <  1:567269/90‑1 (MQ=255)                                                                 ttttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                      <  2:14126/90‑1 (MQ=255)                                                                 ttttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                      <  2:163229/90‑1 (MQ=255)                                                                 ttttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                      <  1:110636/90‑1 (MQ=255)                                                                 ttttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGCACTGCAAGACAAACTCGAcc                      <  1:112464/90‑1 (MQ=255)                                                                        tgtgCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTg               >  2:556146/1‑90 (MQ=255)                                                                                     tGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGGCCttt  >  2:26995/1‑90 (MQ=255)                                                                                        |                                                                                       ATCTCCTCCACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGGCCTTT  >  NZ_CP009273/874150‑874323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑