Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	991,969	T→G	L144F (TTA→TTC)	ssuA ←	aliphatic sulfonate ABC transporter substrate‑binding protein SsuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	991,969	0	T	G	100.0%	17.1 / NA	7	L144F (TTA→TTC)	ssuA	aliphatic sulfonate ABC transporter substrate‑binding protein SsuA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/3);  total (4/3)

CGCGGGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTG  >  NZ_CP009273/991893‑992054                                                                             |                                                                                      cgcggGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCCGGAAAAGGTtgtgtc                                                                          <  1:430067/90‑2 (MQ=255)                gCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCt                                                           >  1:484575/1‑90 (MQ=255)                           gTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACt                                                >  2:270056/1‑90 (MQ=255)                                ttGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTtgtgtg                                                                          <  1:321421/59‑1 (MQ=255)                                ttGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTtgtgtg                                                                          >  2:321421/1‑59 (MQ=255)                                        cGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTaa                                   >  1:410146/1‑90 (MQ=255)                                                                         gCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTg  <  2:27567/90‑1 (MQ=255)                                                                             |                                                                                      CGCGGGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTG  >  NZ_CP009273/991893‑992054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑