Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	98,192	T→C	I314T (ATT→ACT)	murC →	UDP‑N‑acetylmuramate‑‑L‑alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	98,192	0	T	C	100.0%	38.2 / NA	13	I314T (ATT→ACT)	murC	UDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/2);  total (11/2)

CTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGC  >  NZ_CP009273/98107‑98263                                                                                      |                                                                        cTGAATGCGCCAGGTCGTCATAACGCGCAGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTg                                                                     >  1:513163/1‑90 (MQ=255)   gAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCg                                                                   >  2:291944/1‑90 (MQ=255)         gcCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCt                                                             >  1:148985/1‑90 (MQ=255)                 gtcATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCt                                                     <  2:23480/90‑1 (MQ=255)                  tcATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCtt                                                    >  1:549544/1‑90 (MQ=255)                        cgcgCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAggg                                              >  1:258949/1‑90 (MQ=255)                                  gccgcAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGc                                    <  2:272875/90‑1 (MQ=255)                                        gCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGtttt                              >  2:550243/1‑90 (MQ=255)                                           gcggttgcggttGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGAt                           >  2:151337/1‑90 (MQ=255)                                                           cGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTcc           >  1:164968/1‑90 (MQ=255)                                                            ggAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTacc          >  1:315452/1‑90 (MQ=255)                                                                  gggCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGa    >  2:137404/1‑90 (MQ=255)                                                                    gCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGc  >  1:419356/1‑90 (MQ=255)                                                                                      |                                                                        CTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGC  >  NZ_CP009273/98107‑98263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑