Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,336,374	T→G	F66C (TTT→TGT)	pyrF →	orotidine‑5'‑phosphate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,336,374	0	T	G	100.0%	34.8 / NA	12	F66C (TTT→TGT)	pyrF	orotidine‑5'‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/7);  total (5/7)

TCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGG  >  NZ_CP009273/1336311‑1336459                                                                |                                                                                      tCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTcc                                                             <  1:437163/90‑1 (MQ=255)     cAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGa                                                         <  2:340709/90‑1 (MQ=255)            aTGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGATATcccc                                                  <  2:153634/90‑1 (MQ=255)            aTGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGATATcccc                                                  <  2:45902/90‑1 (MQ=255)               tttACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAac                                               <  1:209529/90‑1 (MQ=255)                                acaGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGt                              >  1:515404/1‑90 (MQ=255)                                 caGTTTGTGCGCGAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTc                             >  2:357673/1‑90 (MQ=255)                                           gcgAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAg                   >  2:149163/1‑90 (MQ=255)                                            cgAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGc                  <  2:515404/90‑1 (MQ=255)                                             gAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCt                 >  1:22693/1‑90 (MQ=255)                                             gAACTTCAACAGCGTGGTTGTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCt                 >  2:562242/1‑90 (MQ=255)                                                            ggTTGTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTgg  <  1:39667/90‑1 (MQ=255)                                                                |                                                                                      TCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGG  >  NZ_CP009273/1336311‑1336459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑