Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,503,965	T→C	D67D (GAT→GAC)	hicB →	type II toxin‑antitoxin system antitoxin HicB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,503,965	0	T	C	100.0%	88.3 / NA	28	D67D (GAT→GAC)	hicB	type II toxin‑antitoxin system antitoxin HicB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/13);  total (15/13)

GCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAG  >  NZ_CP009273/1503879‑1504047                                                                                       |                                                                                   gCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCAc                                                                                 <  1:863/90‑1 (MQ=255)     aaGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTa                                                                             <  1:470666/90‑1 (MQ=255)           cTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAg                                                                       >  1:174139/1‑90 (MQ=255)            tttACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGt                                                                      <  1:563918/90‑1 (MQ=255)               aCTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTAcc                                                                   >  1:189327/1‑90 (MQ=255)                       cATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCg                                                           >  2:68606/1‑90 (MQ=255)                         tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc                                                         <  2:66478/90‑1 (MQ=255)                         tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc                                                         <  2:189327/90‑1 (MQ=255)                         tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc                                                         <  1:69162/90‑1 (MQ=255)                          ttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCg                                                        <  1:78328/90‑1 (MQ=255)                              tttttATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCctc                                                    >  1:449868/1‑90 (MQ=255)                               ttttATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCctct                                                   <  2:292889/90‑1 (MQ=255)                                                  aGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATg                                >  1:399095/1‑90 (MQ=255)                                                   gCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGc                               <  1:573818/90‑1 (MQ=255)                                                     ttATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCtt                             >  1:330302/1‑90 (MQ=255)                                                     ttATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCtt                             >  2:189927/1‑90 (MQ=255)                                                            tttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCCGTTAAATGCTTTTTTACa                      <  1:275846/90‑1 (MQ=255)                                                                ccTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCa                  <  1:40442/90‑1 (MQ=255)                                                                ccTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCa                  <  1:162051/90‑1 (MQ=255)                                                                   tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaa               >  1:580985/1‑90 (MQ=255)                                                                   tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaa               >  2:269360/1‑90 (MQ=255)                                                                   tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaa               >  2:425138/1‑90 (MQ=255)                                                                   tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaa               >  2:552479/1‑90 (MQ=255)                                                                   tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaa               >  1:207995/1‑90 (MQ=255)                                                                    cGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaaa              >  1:116453/1‑90 (MQ=255)                                                                    cGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaaa              >  2:265353/1‑90 (MQ=255)                                                                              tAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCa    <  1:549127/90‑1 (MQ=255)                                                                                gTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAag  >  1:318190/1‑90 (MQ=255)                                                                                       |                                                                                   GCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAG  >  NZ_CP009273/1503879‑1504047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑