Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,547,935	A→G	V54A (GTA→GCA)	adhP ←	alcohol dehydrogenase AdhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,547,935	0	A	G	100.0%	84.3 / NA	27	V54A (GTA→GCA)	adhP	alcohol dehydrogenase AdhP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/10);  total (17/10)

TTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGGCT  >  NZ_CP009273/1547871‑1548018                                                                 |                                                                                    ttttAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGcc                                                            <  1:530336/90‑1 (MQ=255)          ggTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAAc                                                   >  1:302913/1‑90 (MQ=255)          ggTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAAc                                                   >  1:346377/1‑90 (MQ=255)          ggTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAAc                                                   >  2:195641/1‑90 (MQ=255)             gACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATg                                                >  1:124468/1‑90 (MQ=255)                   tGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                                          <  2:124468/90‑1 (MQ=255)                    ggACCGACTTCGGCCAACACACCCATGCCTCCATGGCCCAGAATCGCGACGGTTTGCTCACCAAAATCGCCATTCTTAACATGAAGCTCg                                         >  1:132185/1‑90 (MQ=255)                    ggACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCg                                         >  2:503726/1‑90 (MQ=255)                    ggACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCg                                         >  1:288205/1‑90 (MQ=255)                       cccACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTa                                      <  2:214380/90‑1 (MQ=255)                        ccACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTAt                                     <  1:563053/90‑1 (MQ=255)                        ccACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTAt                                     <  2:30757/90‑1 (MQ=255)                        ccACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTAt                                     <  1:471391/90‑1 (MQ=255)                         cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                                          <  1:7119/84‑1 (MQ=255)                         cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                                          >  2:7119/1‑84 (MQ=255)                         cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATg                                    >  1:488220/1‑90 (MQ=255)                         cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATg                                    >  2:201307/1‑90 (MQ=255)                         cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATg                                    >  2:427987/1‑90 (MQ=255)                         cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATg                                    >  1:242674/1‑90 (MQ=255)                          aCTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGa                                   <  1:427987/90‑1 (MQ=255)                               tGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATac                              <  1:429700/90‑1 (MQ=255)                                    ccacACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATAcaccac                         <  2:346377/90‑1 (MQ=255)                                              gCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCAt               >  1:460469/1‑90 (MQ=255)                                                 ttCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCAtttt            >  2:312314/1‑90 (MQ=255)                                                          cAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGGc   >  1:484150/1‑90 (MQ=255)                                                          cAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGGc   >  2:346978/1‑90 (MQ=255)                                                           aGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGGCt  >  2:523969/1‑90 (MQ=255)                                                                 |                                                                                    TTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGGCT  >  NZ_CP009273/1547871‑1548018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑