Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,560,820	T→C	E193G (GAA→GGA)	dosC ←	diguanylate cyclase DosC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,560,820	0	T	C	100.0%	31.5 / NA	11	E193G (GAA→GGA)	dosC	diguanylate cyclase DosC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/5);  total (6/5)

TAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTTCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATC  >  NZ_CP009273/1560734‑1560902                                                                                       |                                                                                   tAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTcccc                                                                                 >  1:431253/1‑90 (MQ=255)   aaCCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCAt                                                                               >  2:583516/1‑90 (MQ=255)       acaGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGaaa                                                                           >  1:134435/1‑90 (MQ=255)                    aGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTAt                                                              >  2:440126/1‑90 (MQ=255)                                                 aaTCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGtttt                                 >  2:101506/1‑90 (MQ=255)                                                              cagGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGagaa                    <  1:330352/90‑1 (MQ=255)                                                                 gATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGAt                 <  1:95755/90‑1 (MQ=255)                                                                  aTTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTCCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATa                >  2:126797/1‑90 (MQ=255)                                                                      tataGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGAt            <  1:440126/90‑1 (MQ=255)                                                                      tataGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGAt            <  2:134435/90‑1 (MQ=255)                                                                            taataTCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCACTAACGAGAAGATACGATAGtttt      >  1:309411/1‑90 (MQ=255)                                                                                atCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATc  <  1:90623/90‑1 (MQ=255)                                                                                       |                                                                                   TAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTTCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATC  >  NZ_CP009273/1560734‑1560902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑