Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,568,198	A→G	F417S (TTC→TCC)	pqqL ←	insulinase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,568,198	0	A	G	100.0%	66.3 / NA	21	F417S (TTC→TCC)	pqqL	insulinase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/9);  total (12/9)

AACTGCTGCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCAT  >  NZ_CP009273/1568111‑1568279                                                                                        |                                                                                  aaCTGCTGCCATTCTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGAc                                                                                 <  1:25813/90‑1 (MQ=255)   ctgctgCCATTTTTCCGCCAGCCTTTGCACGGTAATTTGCTGCCCCAGACGTTTCGAAAGTTGATACGTCTCTTCCGGCGACAAGGACgg                                                                               <  2:587427/90‑1 (MQ=255)   ctgctgCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACgg                                                                               <  2:374685/90‑1 (MQ=255)   ctgctgCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACgg                                                                               <  2:116365/90‑1 (MQ=255)     gctgcCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCg                                                                             >  1:427195/1‑90 (MQ=255)      ctgcCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGt                                                                            >  1:68643/1‑90 (MQ=255)       tgcCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGta                                                                           <  2:564912/90‑1 (MQ=255)       tgcCATTTTTCCGCCAGACTTTGCACGGAAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGta                                                                           >  1:142654/1‑90 (MQ=255)        gcCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGtat                                                                          <  2:125399/90‑1 (MQ=255)                 ccGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATg                                                                 >  2:431652/1‑90 (MQ=255)                                     aTTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTg                                             >  1:467075/1‑90 (MQ=255)                                            gcCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCa                                      >  1:479400/1‑90 (MQ=255)                                            gcCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCa                                      >  1:167990/1‑90 (MQ=255)                                            gcCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCa                                      >  1:120846/1‑90 (MQ=255)                                                      tttCGAAAGTTGATATGTCTCTTCCGGCGACAAGGAAGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATc                            <  2:348398/90‑1 (MQ=255)                                                       ttCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCAt                                     <  1:315648/80‑1 (MQ=255)                                                       ttCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCAt                                     >  2:315648/1‑80 (MQ=255)                                                          gAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGt                        >  2:327644/1‑90 (MQ=255)                                                            aaGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTc                      <  2:75136/90‑1 (MQ=255)                                                                        ctctTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATc          >  1:155615/1‑90 (MQ=255)                                                                                gCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCAt  >  1:206634/1‑90 (MQ=255)                                                                                        |                                                                                  AACTGCTGCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCAT  >  NZ_CP009273/1568111‑1568279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑